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Table 3 a & b: evaluation of different fixatives for processing S. haematobium (Sh) parasite stages for immunolocalization (IL)

From: Immunolocalization of the 29 kDa Schistosoma haematobium species-specific antigen: a potential diagnostic marker for urinary schistosomiasis

Table 3a

Type of fixative

Duration of treatment of Sh parasite stages

Concentration of fixative

0.10

0.25

0.50

1.00

1.50

2.00

Karnovsky’s fixative

90 min

    

+ (+)

3+ (±)

12 hr

    

+ (+)

2+ (±)

Paraform-aldehyde

90 min

      

12 hr

      

Glutar-aldehyde

90 min

  

3 + (2+)

3 + (2+)

2 + (2+)

+ (±)

12 hr

  

3 + (2+)

2 + (2+)

2 + (2+)

± (±)

Methanol

90 min

+ (±)

± (±)

    

12 hr

+ (±)

± (±)

    

Ethanol

90 min

± (±)

+ (±)

    

12 hr

+ (±)

± (±)

    

Acetone

5 min

      

90 min

2+ (−)

2+ (−)

    

12 hr

2+ (−)

2+ (−)

    

Table 3b

Type of fixative

Duration of treatment of Sh parasite stages

Concentration of fixative

  

2.50

3.00

4.00

5.00

10.00

100.00

Karnovsky’s fixative

90 min

+ (+)

     

12 hr

+ (+)

     

Paraform-aldehyde

90 min

 

± (−)

± (−)

   

12 hr

 

+ (−)

+ (−)

   

Glutar-aldehyde

90 min

+ (±)

± (±)

    

12 hr

± (±)

± (±)

    

Methanol

90 min

      

12 hr

      

Ethanol

90 min

      

12 hr

      

Acetone

5 min

     

2+ (−)

90 min

      

12 hr

      
  1. Results in parenthesis, ( ), indicate the intensity of background staining.
  2. ± Indicates weak fluorescence.
  3. Blank spaces on the table represent negative fluorescence and background staining results. Except for 100% acetone which gave positive fluorescence at 5 min of fixation, all the reagents at all the concentrations gave negative fluorescence and background staining at 5, 30 and 60 min of fixation, and were not represented on the table.