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Table 2 Phenotypic and molecular results from whole blood samples and paired BC bottles

From: Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR

BC Sample (BACTEC)

Whole blood sample (EDTA)

Specie isolated from BC bottle (Phoenix®)

Susceptibility profile (Phoenix®)

qPCR from BC bottles for resistance genes (ct)

qPCR from whole blood for resistance genes (ct)

195

176

S. aureus

Oxacillin S

mecA (21,855)

mecA (33,021)

178

117

a

a

ND

mecA (34,520)

190

152

E. coli

Carbapenem S

ND

ND

191

174

P. aeruginosa

Carbapenem S

ND

ND

283

133

E. cloacae

Carbapenem S

ND

ND

391

255

P. putida

Carbapenem S

ND

ND

404

232

Enterococcus sp

Vancomicyn S

ND

ND

341

233

S. aureus

Oxacillin R

mecA (28,550)

mecA (31,653)

371

240

B. cepacia

Carbapenem S

ND

ND

400

252

B. cepacia

Carbapenem S

ND

ND

477

288

S. epidermidis

Oxacillin R

mecA (23,914)

mecA (33,211)

389

237

E. coli

Carbapenem S

ND

ND

369

244

SCoN

Oxacillin S

ND

mecA (33,073)

372

247

P. aeruginosa

Carbapenem S

ND

ND

373

254

S. epidermidis

Oxacillin R

mecA (15,683)

mecA (29,909)

392

241

S. haemolyticus

Oxacillin R

mecA (20,648)

mecA (37,468)

431

276

SCoN

Oxacillin R

mecA (15,902)

mecA (37,320)

465

293

P. aeruginosa

Carbapenem R

ND

ND

441

273

S. epidermidis

Oxacillin R

ND

ND

422

264

S. aureus

Oxacillin R

mecA (16,508)

mecA (35,945)

423

265

SCoN

Oxacillin R

mecA (15,513)

mecA (36,217)

470

290

E. faecium

Vancomicyn R

vanA (17,560)

vanA (24,938)

  1. BC Bloodculture bottle, ct cycle threshold, R resistant, S Susceptible, ND Not detected
  2. aNegative sample at Phenotypic tests