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Table 3 Comparison of smear, culture, single step mPCR and nMPCR for detection of Mycobacterium spp. in clinical samples

From: Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments

Nature of samples

No. of cases

CRS positive cases

Z-N smear (%)

Culture (%)

Single step mPCR (%)

nMPCR (%)

IS6110 & MTP40 (%)

MTP40 & 32kD α-antigen

Only IS6110 (%)

Only MTP40 (%)

Only 32kD α-antigen (%)

IS6110 + MTP40+ 32kD α-antigen

Pulmonary samples

535

415 (77.6)

287 (53.6)

329 (61.5)

345 (64.5)

403 (75.3)

373 (69.7)

6 (1.1)

5 (0.9)

7 (1.3)

9 (1.7)

3 (0.6)

Sputum

523

409 (78.2)

283 (53.3)

323 (61.7)

337 (64.4)

394 (75.3)

365 (69.8)

6 (1.1)

4 (0.8)

7 (1.3)

9 (1.7)

3 (0.6)

BAL

12

6 (50)

4 (33.3)

6 (50)

8 (66.6)

9 (75)

8 (66.6)

0

1 (0.2)

0

0

0

Extra-pulmonary samples

65

35 (53.8)

15 (23.1)

19 (29.2)

20 (30.8)

32 (49.2)

22 (33.8)

1 (1.5)

2 (3.0)

2 (3.0)

4 (6.1)

1 (1.5)

Pleural Fluid

5

3 (60)

2 (40)

3 (60)

3 (60)

3 (60)

3 (60)

1

0

0

0

0

CSF

9

6 (66.7)

3 (33.3)

4 (44.4)

4 (44.4)

5 (55.5)

4 (44.4)

0

0

1 (11.1)

0

0

Bone marrow

1

1 (100)

0

1 (100)

1 (100)

1 (100)

1 (100)

0

0

0

0

0

Pus

15

12 (80)

6 (40)

7 (46.7)

6 (40)

12 (80)

7 (46.7)

1 (6.7)

1 (6.7)

1 (6.7)

1 (6.7)

1 (6.7)

Urine

20

4 (20)

0

0

1 (5)

3 (15)

3 (15)

0

0

0

0

0

FNAs

15

9 (60)

4 (26.7)

4 (26.7)

5 (33.3)

8 (53.3)

4 (26.7)

0

1 (6.7)

0

3 (20)

0

Total

 

450

302 (67.1)

348 (77.3)

365 (81.1)

435 (96.7)

395 (87.8)

7 (1.5)

7 (1.5)

9 (2.0)

13 (2.9)

4 (1.0)

Control

150

0

-

-

-

2 (1.3)

2 (1.3)

-

-

-

-

-

Total

600

-

-

-

-

437 (72.8)

397 (66.2)

-

-

-

-

-

  1. BAL bronchoalveolar lavage, CSF cerebrospinal fluid, FNAs fine needle aspirates, mPCR multiplex PCR, nMPCR nested multiplex PCR