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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Enrichment of bacterial DNA for the diagnosis of blood stream infections

Fig. 2

Efficiency of human DNA removal and sensitivity of PCR assay. a Residues of human DNA after MCLB-1 treatment were monitored via beta-globin derived amplification assays. Upper panel gel based PCR assay targeting the beta-globin gene; lower panel is SYBR green based real-time PCR to quantify residual beta-globin gene fragments. b Detection limits of spiked E. coli at various densities. At high density of spiked bacteria (1000 CFU/ml or 10000 CFU/ml), removal of human DNA does not provide a significant diagnostic signal, but at low concentration (100 CFU/ml or 10 CFU), removal of human DNA enhances detection limit of the diagnostics PCR to 10 CFU/ml

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