Fig. 2From: Enrichment of bacterial DNA for the diagnosis of blood stream infectionsEfficiency of human DNA removal and sensitivity of PCR assay. a Residues of human DNA after MCLB-1 treatment were monitored via beta-globin derived amplification assays. Upper panel gel based PCR assay targeting the beta-globin gene; lower panel is SYBR green based real-time PCR to quantify residual beta-globin gene fragments. b Detection limits of spiked E. coli at various densities. At high density of spiked bacteria (1000Â CFU/ml or 10000Â CFU/ml), removal of human DNA does not provide a significant diagnostic signal, but at low concentration (100Â CFU/ml or 10Â CFU), removal of human DNA enhances detection limit of the diagnostics PCR to 10Â CFU/mlBack to article page