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Table 2 Assay results of the SD BIOLINE HIV/syphilis Duo test for dual detection of HIV and syphilis, Kathmandu, Nepal, 2014

From: Evaluation of SD Bioline HIV/syphilis Duo rapid test kits in Nepal

RDT

Infection

RDT Result

Reference methoda

Positive

Negative

Total

Using SD Bioline HIV/Syphilis duo test kit

Syphilis

Positive

42

13

55

Negative

2

9943

9945

Total

  

10,000

 

HIV

Positive

19

0

19

Negative

0

9981

9981

Total

  

10,000

  1. RDT rapid diagnostic test
  2. aTested by using national HIV testing algorithm [7] with a combination of Determine HIV-1/2/O assay (Abbott Laboratories, Abbott Park, IL), Uni-Gold Recombinant HIV-1/2 (Trinity Biotech, Bray, Ireland) HIV 1/2 Stat-Pak Ultra Fast (Chembio Diagnostic Systems, Medford, NY). Testing for syphilis was done, first by RPR test by using IMMUTREP RPR test kit (Omega Diagnostics Scotland UK) and all test positive samples were re-confirmed by TPHA method. (Thirteen (0.13 %) cases in syphilis testing were found false positive while two were false negative and in case of HIV all of 19 cases tested positive using SD Bioline HIV/Syphilis duo test kit also tested positive by the reference method.)