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Table 2 Results from the “in field” trial: real time amplification mix for different biological fluids were applied on dental mirrors after use, after disinfection in bowl, and after the complete reprocessing procedure

From: Infection control in healthcare settings: perspectives for mfDNA analysis in monitoring sanitation procedures

Used (CT)

Bowl disinfected (CT)

Complete reprocessing (CT)

ID

Mix_Es

Mix_S

Mix_V

Hu DNA

ID

Mix_Es

Mix_S

Mix_V

Hu DNA

ID

Mix_Es

Mix_S

Mix_V

Hu DNA

1u

-

+(36.9)

-

+(28.64)

1d

-

+(39.1)

-

+(27.26)

23

-

-

-

NA

2u

-

+(34.3)

-

+(27.27)

2d

-

+(34.8)

-

+(29.37)

24

-

-

-

NA

3u

-

+(37.8)

-

NA

3d

-

+(37.9)

-

NA

25

-

-

-

NA

4u

-

-

-

+(30.4)

4d

-

+(27.7)

-

NA

26

-

-

-

NA

5u

-

-

-

+(29.9)

5d

-

-

-

NA

27

-

-

-

NA

6u

-

+(36.2)

-

NA

6d

-

-

-

NA

28

-

-

-

NA

7u

-

-

-

+(26.8)

7d

-

-

-

NA

29

-

-

-

NA

8u

-

++(29.1;35.73)

-

+(26.26)

8d

-

++(29.1;35.7)

-

+(30.47)

30

-

-

-

-

9u

-

++(28;30.3)

-

+(26.7)

9d

-

+(36.1)

-

+(27.48)

31

-

-

-

-

10u

-

-

-

+(27.2)

10d

-

-

-

NA

32

-

-

-

-

11u

-

+(38.4)

-

+(31.33)

11d

-

++(31.3;33.1)

-

NA

33

-

-

-

-

12u

-

++(32.7;37.13)

-

NA

12d

-

-

-

NA

34

-

-

-

-

13u

++(30.12;23.21)

+(34.7)

-

NA

13d

-

-

-

NA

35

-

-

-

-

14u

+(32.58)

++(38.29;32.14)

-

+(28.89)

14d

-

+(34.3)

-

+(34.2)

36

-

-

-

NA

15u

+(35.72)

+(33.2)

-

NA

15d

-

+(34.5)

-

NA

37

-

-

-

NA

16u

-

++(35.7;32.5)

-

+(27.21)

16d

-

+(33)

-

+(29.19)

38

-

-

-

NA

17u

-

+(33.2)

-

NA

17d

-

++(35.7;36.8)

-

NA

39

-

-

-

NA

18u

-

++(35.78;36.64)

-

NA

18d

-

+(35.6)

-

NA

40

-

-

-

NA

19u

-

+(34.9)

-

+(27.21)

19d

+(25.2)

+(36.1)

-

+(27.21)

41

-

-

-

NA

20u

-

-

-

-

20d

-

-

-

NA

42

-

-

-

NA

21u

-

+(38.2)

-

NA

21d

-

-

-

NA

     

22u

-

+(38.4)

-

NA

22d

-

-

-

NA

     
  1. (−) negative sample; (+) positive sample; (++) positive sample with two indicators of biological fluid (Mix_ES: E. faecalis and S. aureus; Mix_S: S. salivarius and S. mutans), CT values are shown in brackets; NA = Not Applicable; u: used mirror; d: used mirror after immersion in the disinfectant of the temporary storage bowl; HuDNA: Human DNA; additional testing was performed only on borderline doubt samples selected with a CT over 35