Shedding light on the performance of a pyrosequencing assay for drug-resistant tuberculosis diagnosis

BMC Infectious Diseases

Table 1 Primers Utilized in PCR and Pyrosequencing (PSQ) Reactions

Genes	Target	Forward primer	Reverse primer	Sequencing primer	Detection range	Reference
Mtb Identification	IS6110	Biotin-CCGCCAACTACGGTGTTTA	CAGGCCGAGTTTGGTCAT	GGCCACCTCGATGCC	Multiple	[9]
Isoniazid-R	katG	Biotin-CGGAACCGGTAAGGACGC	CCATTTCGTCGGGGTGTTC	TCCATACGACCTCGAT	Codons 312 to 316	[9]
	inhA	Biotin-ACGCTCGTGGACATACCG	CAGTGGCTGTGGCAGTCA	TGTGGCAGTCACCCC	Position −4 to −20	[9]
	ahpC	TCCTCATCATCAAAGCGGACAAT	Biotin-CGATGCCGATAAATATGGTGTGAT	CATTTGGTTGCGACAT	Position −4 to −23	[9]
Rifampin-R	rpoB1	GGAGGCGATCACACCGCAGACGTT	Biotin-CCTCCAGCCCGGCACGCTCACGT	GCGATCAAGGAGTTCTTC	Codons 507 to 521	[6, 9]
	rpoB2	TTTCGATCACACCGCAGACGTT	Biotin-AAAGGCACGCTCACGTGACAGAC	CAGAACAACCCGCTG	Codons 522 to 533	[6, 9]
Fluoroquinolone-R	gyrA	AATGTTCGATTCCGGCTTCC	Biotin-CGGGCTTCGGTGTACCTCAT	CAACTACCACCCGCAC	Codons 88 to 95	[2]
Injectable-R	rrs	TAAAGCCGGTCTCAGTTCGGAA^aC	Biotin-CAGCTCCCTCCCGAGGGTTA	CTTGTACACACCGCC	Position 1397 to 1406	[9]
Kanamycin-R	eis	Biotin-GGCTACACAGGGTCACAGTC	GCCAGACACTGTCGTCGTAATATTC	CAGACACTGTCGTCG	Position −5 to −47	This study

ISSN: 1471-2334