Skip to main content

Table 6 Distribution of Haemophilus influenzae and Haemophilus parainfluenzae biofilm-producers in patients with sarcoidosis and in healthy people

From: Changes in the prevalence and biofilm formation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory microbiota of patients with sarcoidosis

Category of biofilm producers

OD570 range

No. (%) of isolates

Haemophilus influenzae

Haemophilus parainfluenzae

Healthy people

  

Nasopharynx (n = 1)

 

Nasopharynx (n = 65)

 

Non-producers

0.0

0 (0.0)

 

8 (12.3)

 

Weak

0.01–0.24

1 (100)

 

15 (23.1)

 

Moderate

0.25–0.48

0 (0.0)

 

3 (4.6)

 

Strong

0.49–0.96

0 (0.0)

 

12 (18.5)

 

Very strong

≥0.97

0 (0.0)

 

27 (41.5)

 

Patients with sarcoidosis

  

Nasopharynx (n = 4)

Sputum (n = 8)

Nasopharynx (n = 31)

Sputum (n = 26)

Non-producers

0.0

1 (25.0)

3 (37.5)

12 (38.7)

6 (23.1)

Weak

0.01–0.24

3 (75.0)

5 (62.5)

11 (35.5)

9 (34.6)

Moderate

0.25–0.48

0 (0.0)

0 (0.0)

0 (0.0)

3 (11.5)

Strong

0.49–0.96

0 (0.0)

0 (0.0)

1 (3.2)

4 (15.4)

Very strong

≥0.97

0 (0.0)

0 (0.0)

7 (22.6)

4 (15.4)

  1. Distribution of biofilm-producers was detected using the crystal-violet (CV) method. The cut-off OD (ODc; here: 0.1 ± 0.02) was defined as three standard deviations above the mean OD570 of the negative control. The categories of biofilm-producers: 0 - non-producers (OD ≤ ODc); 1, weak (ODc < OD ≤ 2xODc); 2, moderate (2xODc < OD ≤ 4xODc); 3, strong (4xODc < OD ≤ 8xODc); 4, very strong (8xODc < OD) producers, according to [26]