Skip to main content

Table 1 Single nucleotide polymorphisms (SNPs) included in the genotyping panel

From: Development and validation of a novel single nucleotide polymorphism (SNP) panel for genetic analysis of Blastomyces spp. and association analysis

Gene Region

Function

Category

Variant _ Base pair location

NCBI reference

Chitinase

Carbohydrate metabolism, hyphal growth

Gene

chit_2396

XM_002629522.1

Microsatellite 1.32a

N/A

non-gene region

132GAx11_108

N/Ab

BAD1/WI1

Surface adhesion, modulate host inflammatory response

Virulence genec

BAD1_2556

BAD1_2850

BAD1_2869

U37772.1

ADP-ribosylation factor

Vesicular trafficking

Gene

ARF_374

AY013310.1

ADP-ribosylation factor 6 5′ UTR

N/A

Untranslated region

arf6_240

JN561794.1

histidine kinase

Morphogenesis, cell wall composition, sporulation

Virulence gened

drk1_586e

drk1_595e

JN561950.1

alpha tubulin

Morphogenesis

Gene

TUB1_18

TUB1_277

JN562331.1

orotidine 5′-phosphate decarboxylase

Biosynthesis of pyrimidines

Gene

pyrF_21

pyrF_99

JN562191.1

chitin synthase

Cell Wall/exoskeleton scaffolding

Gene

chs2_203

chs2_290

JN561872.1

fatty acid desaturase

Membrane fluidity, thermotolerance

Gene

fads_622

JN562028.1

internal

transcribed spacer 2 of rDNA

N/A

Gene

ITS2_19e

JN562151.1

urease

Ammonia production, protection from phagocytes

Gene

urease_1503

XM_002623809.1

alpha-[1, 3]-glucan synthase

Cell wall biogenesis, block host recognition

Gene

alpha1_3glucan_2360

alpha1_3glucan_2386

XM_002629303.1

beta-glucosidase

Breaks down cellulose

Gene

b-glucosidase_966

b-glucosidase_1243

XM_002621346.1

septin-1

Filament formation, scaffold, sporulation

Gene

septin1_1251

XM_002628186.1

heat shock protein

Thermotolerance

Gene

hsp_764

XM_002624824.1

DNA Lyase

DNA repair, Flanks mating locus

Gene

APN2_1016

XM_002623165.1

Acetate- coA ligase

Metabolism

Gene

CoAligase_346

XM_002626273.1

tryptophan tRNA ligase

ATP binding

Gene

trypt-lig_922

XM_002620210.1

tyrosinase

Involved in melanin synthesis

Gene

tyrosinase_759

XM_002623880.1

  1. aMicrosatellite locus 5, Meece et al. Applied and Environmental Microbiology 17:5123–5131
  2. bSequence not publically available
  3. cBrandhorst et al. J Biol Chem 275:7925–7934 and Wüthrich et al. Med Mycol 44:41–49
  4. dNemecek et al. Science 312:583–588
  5. eDiagnostic SNPs according to Brown et al. PLoS One 8:e59237