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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Emergence and spread of a new community-genotype methicillin-resistant Staphylococcus aureus clone in Colombia

Fig. 3

Novel structures in the mobile genetic elements in the COL923 clone. Schematic diagrams of the prophage 3, Beta Island and SCCmec J3 region. a Prophage 3 structures identified in COL923 (5sau489), N315 (GenBank number: BA000018.3), NCTC8325 (GenBank number: CP000253.1) and USA300-FPR3757 (GenBank number: CP000255.1). b Beta island structures identified in COL923 (5sau489) and USA300-FPR3757 (GenBank number: CP000255.1). The beta island size in COL923 (5sau489) was 13,980bp. c PCR amplification of a partial region of the beta island in COL923 (5sau489, 20sau42 and Col131 isolates) and USA300-FPR3757. d Structure of the SCCmec J3 regions identified in COL923 (5sau489) and USA300-FPR3757. e PCR amplification of the partial J3 SCCmec region that contains the putative ORF encoding for a transposase (tn) in COL923 (5sau489, 20sau42 and Col131 isolates). Shaded regions represent sequences with >95% of nucleotide identity. SmaI: restriction site for the SmaI enzyme. Black arrows represent the ORFs localized upstream and downstream of the mobile genetic elements and genomic islands. Primers used in PCR amplifications are showed as black triangles

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