Comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and PCR

Pongsachareonnont, Pear; Honglertnapakul, Worawalun; Chatsuwan, Tanittha

doi:10.1186/s12879-017-2264-5

Table 1 Baseline characteristics, culture results, and follow-up data for individual subjects

From: Comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and PCR

Case	Endophthalmitis cause	Age	BCVA	Operation	Gram’s stain	C/S result	H/C result	PCR result	BCVA 1 week	BCVA 1 month	BCVA 3 months
1	trauma	44	HM with PJ	tap	Neg rod	N	Streptococcus Group D	N	S/P evisceration
1	trauma	44	HM with PJ	evisceration	Pos cocci	N	Streptococcus Group D	S. vestibularis	S/P evisceration
2	trauma	50	HM with PJ	tap	N	N	N	N	HM with PJ	20/40	20/20
2	trauma	50	HM with PJ	PPV	N	N	N	machine problem	HM with PJ	20/40	20/20
3	endogenous	46	20/70	PPV	N	N	N	machine problem	HM with PJ	NPL	NPL
4	post-operative	64	HM with PJ	tap	N	N	N	N	FC 1 ft	FC 4 ft	HM with PJ
5	post-operative	59	PL	PPV	N	N	N	H. influenzae	S/P evisceration
5	post-operative	59	PL	evisceration	N	N	N	H. influenzae	S/P evisceration
6	endogenous	71	PJ	PPV	N	Klebsiella pneumoniae	Klebsiella pneumoniae	Klebsiella pneumoniae	S/P evisceration
7	endogenous	72	HM with PJ	PPV	Pos cocci	S. agalactiae	S. agalactiae	S. agalactiae	S/P evisceration
				tap	N	N	S. agalactiae	S. agalactiae
				evisceration	Yeast	N	N	inconclusive
8	trauma	71	HM with PJ	tap	Neg rod	S. pneumonia	N	N	S/P evisceration
8	trauma	71	HM with PJ	evisceration	N	N	N	inconclusive	S/P evisceration
9	post-operative	66	20/70	tap	N	N	Coag. neg. staphylococci	N	FC 1 ft	F/U other hospital
10	post-operative	69	PJ	PPV	N	N	E. faecalis	S. saccharolyticus E. faecalis	PJ	HM with PJ	NPL
				tap	N	N	E. faecalis	S. saccharolyticus E. faecalis
				PPV	N	N	E. faecalis	S. saccharolyticus E. faecalis
11	post-operative	48	FC 1 ft	tap	N	N	N	N	20/30	20/20	loss F/U
11	post-operative	48	FC 1 ft	PPV	N	N	N	N	20/30	20/20	loss F/U
12	post-operative	82	HM with PJ	PPV	N	N	N	N	FC 1/2 ft	FC 1/2 ft	PL
13	post-operative	74	5/200	PPV	N	N	N	E. faecalis	20/30	20/30	20/30
13	post-operative	74	5/200	tap	N	N	N	N	20/30	20/30	20/30
14	post-operative	49	HM with PJ	PPV	Pos cocci	Coag. neg. staphylococci	Coag. neg. staphylococci	S. haemolyticus S. simiae S. aureus	FC 4 ft	20/200	20/200
14	post-operative	49	HM with PJ	PPV	N	N	N	N	FC 4 ft	20/200	20/200
15	trauma	47	HM with PJ	evisceration	N	N	N	N	Post-evisceration
16	endogenous	34	HM with PJ	PPV	N	N	N	N	HM with PJ	HM with PJ	HM with PJ
17	post-operative	35	20/100	tap	N	N	N	N	20/50	F/U other hospital
18	trauma	86	PL	tap	Pos cocci	S. pneumonia	S. pneumonia	N	Post-evisceration
19	trauma	24	FC 1/2 ft	PPV	N	N	N	N	FC 1 ft	FC 1/2 ft	FC 1/2 ft
20	post-operative	49	HM with PJ	PPV	N	N	N	N	20/30	F/U other hospital
21	endogenous	66	HM with PJ	tap	N	N	N	N	HM with PJ	HM with PJ	loss F/U
22	post-operative	50	FC 2 ft	tap	N	N	N	inconclusive	FC 4 ft	20/70	20/70
22	post-operative	50	FC 2 ft	PPV	N	N	N	N	FC 4 ft	20/70	20/70
23	post-operative	72	FC 1 1/2 ft	tap	N	N	N	N	20/70	20/70	20/70
24	endogenous	46	HM with PJ	tap	N	N	N	N	Post-evisceration
25	trauma	54	HM with PJ	PPV	N	N	N	N	Post-evisceration
26	post-operative	64	FC 2 ft	PPV	Neg rod	N	N	N	FC 3 ft	FC 4 ft	FC 6 ft
27	post-operative	74	20/100	tap	N	N	N	N	FC 3 ft	20/200	20/100
28	post-operative	59	HM with PJ	tap	N	N	N	N	HM with PJ	FC ¼ ft
29	trauma	29	PL	PPV	Budding yeast	Curvularia	N	N	HM with PJ

N, no growth of bacteria; inconclusive, growth of multiple bacteria, unable to identified type of bacteria, or possible contamination; machine problem, no result due to machine error; IOFB, intraocular foreign body; phaco, phacoemulsification; IOL, intraocular lens implantation; PPV, pars plana vitrectomy; ECCE, extra-capsular cataract extraction; SBP, scleral buckling procedure; tap, vitreous tap; C/S, conventional bacterial culture method; H/C, blood culture bottle method; PCR, polymerase chain reaction method; F/U, follow up. S.vestibularis, Streptococcus vestibularis; H. influenzae, Haemophilus influenzae; S. agalactiae, Streptococcus agalactiae; S. pneumoniae, Streptococcus pneumoniae, Coag. neg. staphylococci, coagulase-negative staphylococci; E. faecalis, Enterococcus faecalis; S. saccharolyticus, Staphylococcus saccharolyticus; S. haemolyticus, Staphylococcus haemolyticus; S. simiae, Staphylococcus simiae; S. aureus, Staphylococcus aureus; HM, hand motion; PJ, light projection; NPL, no light perception; FC, counting finger; ft, feet; mo, month; Pos cocci, gram positive cocci; Neg rod, gram negative rod

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ISSN: 1471-2334

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