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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

Fig. 1

Anti-N/K type or peptides monoclonal antibody responses examined by enzyme-linked immunosorbent assay (ELISA) or western blotting. a mAb 5D4-C2 reacted to both N-type and K-type Rv0679c equally, as shown by ELISA. b mAb 8G10-H2 reacted to N-type exclusively, as shown by ELISA. c 5D4-C2 recognized the linear peptide (peptide-4) covering residues 31–50 (AGCSSTANPPAATTTPATAT) within 16 consecutive overlapping peptides from 1 to 165 of Rv0679c; * indicates a significant difference in the level of reaction between 5D4-C2-peptide-4 and others, p < 0.05. d, e indicates sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE) western blots and native PAGE western blots, respectively, 5D4-C2 reacted with both N-type and K-type, while 8G10-H2 reacted only with N-type; different migration patterns of N-type and K-type were assessed in native PAGE; BSA, bovine serum albumin

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