Fig. 1

Diagnostic flowchart of congenital Toxoplasma gondii infection. Adapted from Van Haesebrouck et al. [28] and Montoya and Remington [27]. ^#Low avidity can be an indication of recent infection but should not be directly interpreted as recent infection [26]. *Amniotic fluid PCR can be performed starting from 18 weeks of gestation onwards and at least 8 weeks after diagnosis of seroconversion. The risk of the procedure should be carefully weighed against the potential benefit of diagnosing fetal infection [27]. pos, positive; neg, negative; US, ultrasound; PCR, polymerase chain reaction; TGS, Toxoplasma gondii specific