Fig. 2

Laboratory confirmation of T. gondii congenital infection. Left: Western blot analysis of IgG and IgM antibody response of the patient (P) and his mother (M). C+ and C- are positive and negative newborn controls. The arrow indicates a slight but persistent IgM band in the baby (present in the second sample, not shown). Right: Real Time PCR quantification of the parasite load. The blue squares correspond to different dilutions of the maternal blood DNA, while the green ones are from the patient. The standard curve (red squares) is constructed by adding different numbers of RH strain tachyzoites to a negative blood and performing extraction and PCR procedures in parallel to those of the samples