Table 1 Details of vectors, gene primers and host cells used in this study
S.No. | Components used | Name/Sequence | Source |
|---|---|---|---|
1 | Vector | pET-SUMO | Invitrogen |
2 | Host cells | Mach1™-T1R Chemically competent E. coli cells BL21(DE3) chemically competent E. coli. Cells | Invitrogen Sigma |
3 | Gene | omp2a | B. melitensis 16 M genome |
4 | Primer used for omp2a gene amplification | Forward - 5’ATGAACATCAAGAGCCTT3’ Reverse - 5’TTAGAACGAGCGCTG3’ | This study |
5 | T7-promoter reverse sequencing primer of vector | Reverse - 5’TAGTTATTGCTCAGCGGTGG3’ | Invitrogen |