Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus

Table 3 Analytical sensitivity of ZIKV RT-qPCR and RT-iiPCR assays using ZIKV in vitro transcribed RNA

	ZIKV prM RT-qPCR		ZIKV E RT-qPCR		ZIKV NS2B RT-qPCR		ZIKV RT-iiPCR
ZIKV RNA copies/μl	No. positive/No. tested	Rate (%)	No. positive/No. tested	Rate (%)	No. positive/No. tested	Rate (%)	No. positive/No. tested	Rate (%)
10⁷	6/6	100	6/6	100	6/6	100	6/6	100
10⁶	6/6	100	6/6	100	6/6	100	6/6	100
10⁵	6/6	100	6/6	100	6/6	100	6/6	100
10⁴	6/6	100	6/6	100	6/6	100	6/6	100
10³	3/6	50	6/6	100	6/6	100	6/6	100
10²	1/6	17	6/6	100	6/6	100	5/6	83
10	0/6	0	5/6	83	6/6	100	5/6	83
1	0/6	0	1/6	17	1/6	17	1/6	17
0.1	0/6	0	0/6	0	0/6	0	0/6	0

ISSN: 1471-2334