High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil

Table 1 Primers used in polymerase chain reaction analysis

Gene	Description	Primer Sequence (5′- 3′)	PCR product	Reference
aggR	Transcriptional activator	CTAATTGTACAATCGATGTA ATGAAGTAATTCTTGAAT	308 bp	[37]
aap	Antiaggregation protein	CTTTTCTGGCATCTTGGGT GTAACAACCCCTTTGGAAGT	232 bp	[37]
aggA	AAF/I fimbria subunit	TTAGTCTTCTATCTAGGG AAATTAATTCCGGCATGG	450 bp	[37]
aafA	AAF/II fimbria subunit	ATGTATTTTTAGAGGTTGAC TATTATATTGTCACAAGCTC	518 bp	[37]
agg-3A	AAF/III fimbria subunit	GTATCATTGCGAGTCTGGTATTCAG GGGCTGTTATAGAGTAACTTCCAG	462 bp	[38]
had	AAF/IV fimbria subunit	TCCATTATGTCAGGCTGCAA GGCGTTAACGTCTGATTTCC	411 bp	[41]
paa	Porcine AE/associated adhesin	ATGAGGAACATAATGGCAGG TCTGGTCAGGTCGTCAATAC	357 bp
aida/aah	AIDA-I adhesin	TCGATACCGAAACGCATACGCAGA ACGCCGATCGGTGATGATGAAGAT	204 bp
afaE	Afa-I afimbrial adhesin	CGAAAACGGCACTGACAAG AGGCTTTCCGTGAATACAACC	230 bp	[34]
daaE	F1845 fimbrial adhesin	TGACTGTGACCGAAGAGATGC TTAGTTCGTCCAGTAACCCCC	380 bp	[34]
sat	Secreted autotransporter toxin	CTCATTGGCCTCACCGAACGG GCTGGCAGCTGTGTCCACGAG	299 bp
pic	Serine protease precursor	ACTGGCGGACTCATGCTG T AACCCTGTAAGAAGACTGAGC	387 bp
pet	Plasmid-encoded toxin	GACCATGACCTATACCGACAGC CCGATTTCTCAAACTCAAGACC	600 bp
astA	EAST1 heat-stable toxin	CCATCAACACAGTATATCCGA GGTCGCGAGTGACGGCTTTGT	111 bp
chuA/ shuA	Heme receptor	ATCTGCTGCGTCATGTTCCT GTAGTGGTCATACCTTTGAGC	1700 bp
iucA	Aerobactin sintase	AGTCTGCATCTTAACCTTCA CTCGTTATGATCGTTCAGAT	1100 bp
irp2	Iron chelating	AAGGATTCGCTGTTACCGGAC TCGTCGGGCAGCGTTTCTTCT	264 bp

ISSN: 1471-2334