Fig. 2

Comparison of densities of immunoreactive cells and morphological scoring of microglia in 10 piglets suffering from meningitis and 14 control piglets. In sections of the hippocampal dentate gyrus, significantly more apoptotic neurons were found in piglets with meningitis compared to control piglets (in-situ tailing) (a). No significantly different densities of dividing cells (proliferating cell nuclear antigen (PCNA) staining) (b), young neurons (calretinin staining) (c), microglia (ionized calcium binding adaptor molecule 1 (Iba-1) staining) (e) and astrocytes (glial fibrillary acidic protein (GFAP) staining) were detected (f). Morphological scoring of Iba-1 stained cells in the neocortex revealed a significantly stronger activation of microglia in piglets with meningitis (d). *p ≤ 0.05; ***p ≤ 0.001