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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Human monoclonal anti-protective antigen antibody for the low-dose post-exposure prophylaxis and treatment of Anthrax

Fig. 1

a Construction of PA21 expression vectors. M, marker; lane 1, expression vector for PA21 heavy chain; lane 2, linearised pTH; lane 3, variable region of PA21 Fab heavy chain; lane 4, expression vector for PA21 kappa chain; lane 5, linearised pTL; lane 6, variable region of PA21 Fab kappa chain. b Purification of PA21. M, marker; lane 1, PA21 antibody; lane 2, control human IgG; lane 3, PA21 (cell supernatant); lane 4, PA21 (flow through); lane 5, 293F cell supernatant

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