Fig. 4

BALB/c mice produce infectious HEV viruses upon inoculation of capped RNA transcripts of the swine HEV clone. A549 cells were infected with the feces suspension of BALB/c mouse inoculated with HEV RNA transcripts. Cells were harvested at 72 h post-inoculation to detect ORF2 protein by Western blotting. The feces suspension of swine HEV served as positive control. GAPDH served as a loading control