Fig. 1

Cytokine levels in culture supernatant of healthy and leprosy patients. Cytokine levels in culture supernatant of unstimulated (A) M. leprae antigens stimulated (B) PBMCs of healthy individuals (H), TT/BT and BL/LL patients. PBMCs from the study subjects were cultured with or without antigens for 5 days in RPMI at 37 °C in 5% humidified atmosphere. Supernatants were collected after 48 h and 5 days of culture and cytokine was estimated. Concentration of cytokine was normalized after subtracting values of unstimulated supernatant from stimulated supernatant. I = IFNγ, II = IL-4, III = IL-10. * = p < 0.05, ** = p < 0.001. MLSA- M. leprae soluble antigen, WCL- whole cell lysate, PGL-1- Phenolic glycolipid-1. BT/TT (Borderline tuberculoid/Tuberculoid), BL/LL (Borderline lepromatous/Lepromatous)