Fig. 3

IDO1, TGF-β1, FoxP3 and IFN-γ expression levels as a response to C. trachomatis infection, with or without azithromycin treatment. Transcript levels of IDO1, TGF-β1, FoxP3 and IFN-γ (2^{- ΔCT}) were measured from cell cultures of (a) human endometrial cell line ECC1, (b) PBMCs of C. trachomatis negative female, and (c) co-culture of ECC1 and PBMCs. Transcript levels were compared between treatments; without Chlamydia infection or azithromycin treatment (−CT –AZ), no Chlamydia infection with azithromycin (−CT + AZ), with Chlamydia infection no azithromycin (+CT –AZ) and with Chlamydia infection and azithromycin treatment (+CT + AZ). Cells were infected with C. trachomatis at MOI of 0.1. Azithromycin was added to the culture at 20 h PI, or at 44 h post seeding in control treatments. Total RNA was isolated from cultures at 44 h PI. Data are presented as mean ± SD. Significant differences are indicated in the graph (p < 0.05), using 2-way ANOVA with Dunnett’s multiple comparison post-test