Alterations in bacterial communities, SCFA and biomarkers in an elderly HIV-positive and HIV-negative population in western Mexico

Table 1 Information of the oligonucleotides used for the qPCR analysis

Primer	Sequence (5′-3′)		Temp. (°C)	Prod. size (bp)	Reference
16S rRNA	16S-F	AGTTTGATCCTGGCTCAG	62	500	González et al.; 2012
16S rRNA	16S-R	GWATTACCGCGGCKGCTG	62	500	González et al.; 2012
Bacteroidetes	Bact-F	GGARCATGTGGTTTAATTCGATGAT	63	126	Guo et al.; 2008
Bacteroidetes	Bact-R	AGCTGACGACAACCATGCAG	63	126	Guo et al.; 2008
Firmicutes	Firm-F	GGAGYATGTGGTTTAATTCGAAGCA	63	126	Guo et al.; 2008
Firmicutes	Firm-R	AGCTGACGACAACCATGCAC	63	126	Guo et al.; 2008
Proteobacterias	Prot-F	TCGTCAGCTCGTGTYGTGA	56	170	Bacchetti De Gregoris et al.; 2011
Proteobacterias	Prot-R	CGTAAGGGCCATGATG	56	170	Bacchetti De Gregoris et al.; 2011
Actinobacterias	Acti-F	TACGGCCGCAAGGCTA	57	170	Liang et al.; 2016
Actinobacterias	Acti-R	TCRTCCCCACCTTCCTCCG	57	170	Liang et al.; 2016
Lactobacillus (genero)	Lacto-F	GCGGTGAAATTCCAAACG	56	216	Hermann et al.; 2013
Lactobacillus (genero)	Lacto-R	GGGACCTTAACTGGTGAT	56	216	Hermann et al.; 2013
Bifidobacterium	Bifi-F	CGGGTGAGTAATGCGTGACC	56	139	Furet et al.; 2009
Bifidobacterium	Bifi-R	TGATAGGACGCGACCCCA	56	139	Furet et al.; 2009
C. leptum	Clep-F	CCTTCCGTGCCGSAGTTA	60	115	Furet et al.; 2009
C. leptum	Clep-R	GAATTAAACCACATACTCCACTGCTT	60	115	Furet et al.; 2009
C. coccoides	Ccoc-F	GACGCCGCGTGAAGGA	56	199	Furet et al.; 2009
C. coccoides	Ccoc-R	AGCCCCAGCCTTTCACATC	56	199	Furet et al.; 2009

ISSN: 1471-2334