Fig. 4

Representative flow plots for cytokine staining and cytotoxic molecules of NK cells. Lymphocyte gate (A). Selected NK cells (CD3⁻CD56⁺) (B). Representative flow plots showing expression of IFN-γ (C), (CD107a (D), granzyme B (E) and perforin (F) by NK cells from one representative individual with HAM/TSP, an asymptomatic HTLV-1-infected individual (AS) and one uninfected control (CTR), cultured in the presence of K562 for 6 h. Pink dot plots represent specific–stained population and blue dot plots represent background fluorescence observed with matched isotype control. Results reflect one representative experiment out of six independent experiments performed