Table 1 Primer sequences used in this study for detection of Anaplasma species pathogenic to humans
Pathogens | Target gene | Primer | Oligonucleotide sequences (5′- 3′) | References |
|---|---|---|---|---|
A. phagocytophilum | rrs | EE1 | TCCTGGCTCAGAACGAACGCTGGCG (+) | Barlough et al. [22] |
|  |  | EE2 | AGTCACTGACCCAACCTTAAATGGCTG (−) | Barlough et al. [22] |
| Â | Â | SSAP2f | GCTGAATGTGGGGATAATTTAT (+) | Kawahara et al. [23] |
| Â | Â | SSAP2r | ATGGCTGCTTCCTTTCGGTTA (+) | Kawahara et al. [23] |
A. capra | rrs | Â | GCAAGTCGAACGGACCAAATCTGT (+) | Yang et al. [26] |
|  |  |  | CCACGATTACTAGCGATTCCGACTTC (−) | Yang et al. [26] |
| Â | Â | Ehr3 | TGCATAGGAATCTACCTAGTAG (+) | Rar et al. [28] |
|  |  | Ehr4 | CTAGGAATTCCGCTATCCTCT (−) | Rar et al. [28] |
A. ovis | rrs | EE1 | TCCTGGCTCAGAACGAACGCTGGCG (+) | Barlough et al. [22] |
|  |  | EE2 | AGTCACTGACCCAACCTTAAATGGCTG (−) | Barlough et al. [22] |
| Â | Â | 297E | ACACGGTCCAGACTCCTACG (+) | Ochirkhuu et al. [24] |
|  |  | 1144R | CTTGACATCATCCCCACCTT (−) | Ochirkhuu et al. [24] |
A. platys | rrs | PLATYS | GATTTTTGTCGTAGCTTGCTATG (+) | Martin et al. [27] |
|  |  | Ehr2 | AGTAYCGRACCAGATAGCCGC (−) | Rar et al. [28] |
|  |  | Ehr4 | CTAGGAATTCCGCTATCCTCT (−) | Rar et al. [28] |