Table 2 Primer sequences designed to amplify the gltA and groEL genes in this study
Pathogens | Target gene | Oligonucleotide sequences (5′- 3′) | Fragment |
|---|---|---|---|
A. platys | gltA (partial) | Pglt-F: ATGAWAGAAAAWGCTGTTTT (+) | / |
|  |  | Pglt-R1: TCATGRTCTGCATGCATKATG (−) | |
|  |  | Pglt-R2: CATGCATKATGAARATMGCAT (−) | |
| Â | groEL (partial) | Pgro-F1: TTGATCATCGCTGAAGACGT (+) | / |
| Â | Â | Pgro-F2: ACTCTCGTCTTGAACAAGCT (+) | |
|  |  | Pgro-R: CCACTCTGTCTTTACGCTCT (−) | |
| Â | gltA (nearly complete) | Pglt-F: ATGAWAGAAAAWGCTGTTTT (+) | Former |
|  |  | Pglt-R1: TCATGRTCTGCATGCATKATG (−) | |
|  |  | Pglt-R2: CATGCATKATGAARATMGCAT (−) | |
| Â | Â | Pglt-L-F1: GATGCWCATCCYATSGCMATGT (+) | Latter |
| Â | Â | Pglt-L-F2: CGTGMTSGCTATAGCGMAART (+) | |
|  |  | Pglt-L-R: TCAYACCATTGDGAYRCCCAT (−) | |
| Â | groEL (nearly complete) | Pgro-F-F: AAATGKCAAATACGGTWGTC (+) | Former |
|  |  | Pgro-F-R1: ACAACACCTTCCTCKACAGC (−) | |
|  |  | Pgro-F-R2: CTGKCTTTRCGYTCTTTAACTTC (−) | |
| Â | Â | Pgro-L-F1: GAYGGTATGCAGTTTGATCGCG (+) | Latter |
| Â | Â | Pgro-L-F2: ATGCAGTTTGATCGCGGWTATC (+) | |
|  |  | Pgro-L-R: CAGCRAGGTCGAAYGCAATAC (−) |