Table 1 Components of PCR master mix
From: A highly sensitive modified nested PCR to enhance case detection in leishmaniasis
PCR water | 19.8 μl |
|---|---|
2XPCR Taq master mix (abm, G013) | 25.0 μl |
Forward, outer primer (P221) | 0.1 μl |
Reverse, outer primer (P332) | 0.1 μl |
Template DNA | 5.0 μl |
Total | 50.0 μl |