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Table 3 Details of items used for evaluating the per patient cost of Mo-STNPCR

From: A highly sensitive modified nested PCR to enhance case detection in leishmaniasis

Major steps of Mo-STNPCR

Considered items for per patient cost analysis of Mo-STNPCR

Sample collection

Sample collection tubes and other materials required for sample collection

DNA extraction

DNA extraction kits for sample, positive control and negative control

PCR procedure

PCR water, 2XPCR Taq master mix (abm, G013), outer primers (P221 and P332) and inner primer (P223 and P333), bromophenol blue

PCR product analysis

Gel running buffer, ethidium bromide, agarose

Other consumables and reagents

Micro-centrifuge tubes, PCR tubes, pipette tips, phosphate buffered saline, issuing laboratory reports