From: A highly sensitive modified nested PCR to enhance case detection in leishmaniasis
Major steps of Mo-STNPCR | Considered items for per patient cost analysis of Mo-STNPCR |
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Sample collection | Sample collection tubes and other materials required for sample collection |
DNA extraction | DNA extraction kits for sample, positive control and negative control |
PCR procedure | PCR water, 2XPCR Taq master mix (abm, G013), outer primers (P221 and P332) and inner primer (P223 and P333), bromophenol blue |
PCR product analysis | Gel running buffer, ethidium bromide, agarose |
Other consumables and reagents | Micro-centrifuge tubes, PCR tubes, pipette tips, phosphate buffered saline, issuing laboratory reports |