Fig. 2
From: Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae
Allelic discrimination of the ASPCR assays. A: ASPCR assays of A2063G. B: ASPCR assays of A2064G. Ct value comparisons of the serial dilutions (106–101 copies) of mutant DNA with 105 copies of wild-type DNA (open squares) and the mutant DNA without the addition of wild-type DNA (solid symbols). The addition of non-complementary wild-type templates to the mutant standard did not significantly alter the Ct values until the mutant DNA was 10 copies in the reaction. The Ct value of 105 copies of wild-type WT template amplified by A2063G and A2064G specific primers was 32.78 ± 0.26 and 31.75 ± 0.34, respectively