Fig. 1

Genome coverage and phylogenetic analysis of Toscana virus imported to Switzerland. a) Genome coverage of Toscana virus (TOSV) segments S, M and L by metagenomic sequencing analysis. The black bar below segment S shows the region sequenced by specific PCR. b) Phylogenetic analysis of the full-length nucleotide sequence of TOSV segment S. The isolate reported in this study, 1000414117 (MK422500), belongs to lineage B and is marked with a closed circle, a previous isolate imported to Switzerland with an open circle (GU270841, partial coding sequence only). Phylogenetic analysis was performed in MEGA7 using the Maximum Likelihood method based on the Kimura 2-parameter model. All positions with less than 50% site coverage were eliminated