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Table 4 Excluded full texts and the reasons for exclusion

From: Sensitivity and specificity of diagnostic tests for Lassa fever: a systematic review

Study

Assay

Reason

1- Fukuma et al, 2011 [27]

Reverse Transcription LAMP

No data on sensitivity and specificity. Just talks about assay development

1- Fukushi et al, 2012 [28]

Serological assays

Review. No data on performance

3- Pang et al, 2014 [29]

Multiplex one step Real-Time Taqman qRT-PCR

No data on sensitivity and specificity given. Not a lab or field evaluation.

4- Das et al, 2015 [30]

Multiplex PCR/LDR assay

Not an evaluation. No data on sensitivity and specificity

5- Jones A et al, 2011 [31]

Handheld multiplex POC diagnostics

No data on sensitivity and specificity

6- Trombley et al, 2010 [32]

RT-TaqMan PCR

Not an evaluation study. No data on sensitivity and specificity

7- Drosten et al, 2002 [33]

SYBR-green real time RT-PCR

Not a lab or field evaluation.

8- Bukbuk et al, 2014 [34]

Recombinant NP(rNP) IgG ELISA

No data on sensitivity and specificity

9- Barber et al, 1990 [35]

Recombinant ELISA

Not evaluated in humans. No data on sensitivity and specificity

10- Li et al, 2009 [36]

Fluorescent microbeads based multiplex assay

No data on performance

11- Saijo et al, 2007 [37]

Ag-Capture ELISA

No data on the performance

12- Salvato et al, 2018 [38]

 

Review

13- Satterly et al, 2016 [39]

Ag and IgM Capture(MAGPIX)

 

14- Olschlager et al, 2012 [40]

RT-PCR/hybridization assay

No data on sensitivity and specificity

15- Vieth et al, 2007 [41]

RT-PCR

Not an evaluation study

16- Leski et al, 2009 [42]

High Density Resequencing microarray

No data on sensitivity and specificity.

17- O’Hearn et al, 2016 [43]

IgG(MAGPIX)

No data on sensitivity and specificity

18- Demby et al, 1994 [44]

Standard RT-PCR

Not an evaluation study

19- Sebba D et al, 2018 [45]

Multiplex POC test for Lassa, Ebola and Malaria

None of the 276 clinical samples tested with the 3-plex assay were independently tested for LASV.

20- Koehler et al, 2018 [46]

Highly Multiplex Broad Pathogen detection assay

No data on sensitivity and specificity.