- Research article
- Open Access
Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon
BMC Infectious Diseases volume 20, Article number: 32 (2020)
Vaginal candidiasis is frequent in pregnant women and is associated with sepsis and adverse neonatal outcomes. This study determined the prevalence of candida species in symptomatic pregnant women and evaluated the antifungal susceptibility profile of the isolated Candida strains. It also aimed to explore whether Candida species predicts gestational complications and adverse neonatal outcomes.
A total of 258 pregnant women with vaginal discharge at 35 to 37 week of gestation participated in this study. Vaginal swabs from these patients were collected at various obstetrics and gynecology clinics in Lebanon for a period of 14 months. Candida isolates were identified at species level and antifungal susceptibility of Candida albicans to fluconazole (FCZ), amphotericin B (AMB), itraconazole (ICZ) and voriconazole (VCZ) was determined by the agar-based E-test method.
Among 258 women tested, 100 (39%) were positive for Candida species. C. albicans, C. glabrata and C. krusei were isolated from 42, 41 and 17% of the women, respectively. C. albicans was significantly associated only with gestational diabetes while C. krusei or C. glabrata had significant positive associations with other gestational complications. The antifungal susceptibility tests of C. albicans isolates revealed 97.5, 90, 87.5 and 97.5% susceptibility to AMB, FCZ, ICZ and VCZ, respectively.
The current study revealed high incidence of both C. albicans and non-C. albicans Candida strains causing vulvovaginitis among pregnant women in Beirut, Lebanon. Candida screening as antenatal follow up is advised to minimize the risk of adverse neonatal outcome or gestational complications.
Candida species, which are part of the normal flora in the vulvovagina, may cause opportunistic infections under various circumstances that compromise host immunity. Candida spp. subsist in symbiotic relationship with vaginal microbiota, therefore asymptomatic colonization is common and may persist for years. The rate of genital Candida colonization ranges from 20% in asymptomatic young women to up to 30% in pregnant women [1,2,3,4,5,6]. The risk factors associated with increased rate of vulvovaginal candidiasis (VVC) in pregnant women are immunologic alterations, increased estrogen levels, and increased vaginal glycogen production mechanism .
VVC is the result of Candida albicans in 85–95% of cases whereas incidence rate of Non-C. albicans Candida (NCAC) in pregnant women and non-pregnant women is less than 10% as described in previous studies .
Treatment of VVC is recommended only in the presence of symptoms since over 20% of women may have yeast as part of their natural vaginal microbiome and are asymptomatic . However, pregnant women may have severe and prolonged symptoms of VVC requiring longer courses of therapy . Recent studies have shown an increase in the development of drug-resistance among C. albicans, less is known about the burden and effects of drug resistant fungal infections.
Candidiasis in newborns has been associated with increased risk of pregnancy complications, such as premature rupture of membranes, preterm labor, chorioamnionitis, and congenital cutaneous candidiasis. Colonization with Candida spp. in neonates may occur by vertical transmission from the mother during the perinatal period or by horizontal transmission in the nursery or the neonatal intensive care unit (NICU) [10,11,12]. It has been shown that 5 to 30% of all colonized preterm neonates will develop invasive Candida infection (ICI) during their stay in the NICU [13,14,15]. C. albicans was shown to play a major role in neonatal colonization in the first days of life and were also documented in a group of premature infants .
The epidemiology of antifungal resistance among C. albicans in pregnant women in Lebanon remains poorly reported. Therefore, the objective of this study was to determine the prevalence of Candida species in symptomatic pregnant women with vaginal discharge at 35 to 37 weeks of gestation and to evaluate the antifungal susceptibility profile of the isolated strains of C. albicans. In addition, the study evaluated the association between the presence of Candida species and gestational complications and outcomes.
Study sample and procedure of collection
In this study, a cross-sectional design was adopted for determining the prevalence of Candida species in Lebanese pregnant women. Clinical samples were collected from 258 pregnant women with vaginal discharge in three obstetrics and gynecology clinics in Lebanon during a period of 14 months (June 2015–July 2016). Women were approached by a registered gynecologist who explained the objectives of the study and asked them to participate. Participation was voluntary and anonymous. Two vaginal swabs were collected from each patient. The samples were stored in Stuart media (Oxoid, UK) at room temperature and transported to the clinical diagnostic laboratory.
Socio-demographic data, clinical status and gestational history of 165 (64%) patients were collected through a questionnaire that included information about mothers’ risk factors for adverse neonatal outcomes such as gestational diabetes, previous miscarriage, anemia and recurrent urinary tract infections (UTI). The 165 participants were followed up after delivery to gather information about delivery time, delivery type, induced labor, gestational complications (intrahepatic cholestasis, mitral valve prolapse, asthma, hypothyroidism, oligohydramnios and gestational thrombocytopenia) and neonatal outcomes (newborn height, weight and apgar score).
To compare the distribution of Candida species isolated from women genital tract in different countries, a Pubmed search was performed that included articles published in the last 10 years [17,18,19,20,21,22,23]. Fifteen articles were selected and summarized in Fig. 1.
Culture and identification
Vaginal swabs were cultured on both Sabouraud dextrose agar (SDA) and Chromatic Candida medium (Liofilchem, Italy). The latter allows the selective isolation and differentiation of Candida spp. based on colony color and morphology; it has been well documented in previous studies as for its high sensitivity and specificity for the identification of the most commonly encountered Candida spp. [24,25,26]. Both plates were incubated at 37 °C for 48 to 72 h. The chromatic characteristics of the colonies were the following: green colonies were identified as C. albicans, creamy colored colonies as C. glabrata, and pink with a whitish border colonies as C. krusei. All isolates were confirmed by API 20 C AUX strip (BioMerieux, Marcy l’Etoile, France). Further phenotypic testing was done to differentiate between C. dubliniensis and C. albicans by growing the germ tube positive yeast isolates at 45 °C on SDA for up to 10 days. C. albicans isolates were identified by their ability to grow at 45 °C.
Antifungal susceptibility testing
The in vitro activity of the antifungal agents against each isolate was determined by the E-test (HiMedia, Mumbai, India) in accordance with the manufacturer’s instructions. The E-test strips of fluconazole (FCZ; 0.016 ∼ 256 μg/mL), itraconazole (ICZ; 0.002 ∼ 32 μg/mL), voriconazole (VCZ; 0.002 ∼ 32 μg/mL), and amphotericin B (AMB; 0.002 ∼ 32 μg/mL) were used . Interpretive susceptibility criteria for antifungal breakpoints were adapted from the Clinical and Laboratory Standards Institute (CLSI), 2017 (21). The breakpoints used for C. albicans are: FCZ (S ≤ 2; SDD = 4; R ≥ 8); VCZ (S ≤ 0.12; R ≥ 1), ICZ (S ≤ 0.12; R ≥ 1) and AMB (S ≤ 2; R > 2). For quality control, C. albicans (ATCC 10231) was used as reference strain and tested simultaneously with the clinical isolates.
The presence of Candida species was correlated with the newborn height, weight and Apgar score (overall assessment of new born well-being used immediately following the delivery of the baby) as dependent variables using linear multiple regression analysis. The models took into consideration to control other possible confounding effect of various independent variables including mother’s age, mother’s education, delivery type, delivery week, induced labor, recurrent urinary tract infection (UTI), gestational diabetes, anemia and other gestational complications. Statistical significance was calculated using p-value and confidence intervals. The presence of Candida species effect on categorical outcome variables (Gestational diabetes, vaginal discharge, induced labor and recurrent UTI) was explored by comparing frequencies using the test of independence Chi-square. P values were computed considering p ≤ 0.05 as significant results.
The socio-demographic characteristics of 165 respondents are summarized in Table 1; 49% of women who participated were between the ages of 36 to 40 years old. The majority completed their university degree (62.4%). The rate of normal vaginal delivery was 43.6 and 69.7% of women had labor induction. Gestational complications occurred in 59.1% of women and 25.5% of women had gestational diabetes mellitus. Anemia was reported in 14.5% of women.
The data presented in the table are number (%) of patients. Missing are missing data since patients’ information were not provided; those were excluded from the analysis. Gestational complications include intrahepatic cholestasis, mitral valve prolapse, asthma, hypothyroidism, oligohydramnios and gestational thrombocytopenia.
Among the cultures from the 258 women tested, 100 (39%) were positive for Candida species.
C. albicans was isolated from 42% of the women (N = 42) and NCAC from the remaining 58%. Figure 1 summarizes the distribution of Candida species isolated from vaginal swabs from population-based studies conducted in different countries including our study over the last decade. China, Brazil, Tunis, Kuwait, India, Greece and Turkey have reported that C. albicans remains the most commonly isolated yeast (60–80%) in women diagnosed with VVC [17,18,19,20,21,22,23]. On the other hand, an increasing trend in the occurrence of NCAC (58–60%) over time has also been observed in Pakistan and Burkina Faso [28, 29]. The main identified NCAC in our study were C. glabrata (71%, N = 41) followed by C. krusei (29%, N = 17). Four women were co-infected with both C. albicans and C. glabrata. All of the three identified Candida species were isolated from women in the age group 30–40 years.
The observed susceptibility rates of C. albicans isolates to AMB, FCZ, ICZ and VCZ were 97.5, 90, 87.5 and 97.5%, respectively. MIC50 and MIC90 of the antifungal agents tested against 40 strains of C. albicans are presented in Table 2. Two of the isolates were lost during processing, Although ICZ presented the lowest MIC90 value of 0.125 μg/mL, it showed highest resistance rate (12.5%) among all the agents tested.
MIC50 = Minimum Inhibitory Concentration required to inhibit the growth of 50% of organisms. MIC90 = Minimum Inhibitory Concentration required to inhibit the growth of 90% of organisms. MIC range is the range of the lowest and highest MIC values obtained from 40 C. albicans isolates tested. Percentage resistance is the percentage of isolates resistant to a specific antifungal drug.
The association between the presence of Candida species, isolated from 48 women who filled the questionnaire, was assessed with preterm delivery, delivery type, gestational complications, gestational diabetes, recurrent UTI infection and induced labor (Table 3). C. albicans was significantly associated only with patients with gestational diabetes; 33% of C. albicans positive and 24% of NCAC participants had gestational diabetes (p = 0.04). C. albicans had non-significant associations with gestational complications, induced labor and recurrent UTI. On the other hand, the presence of C. krusei and C. glabrata had strong significant association with premature delivery and gestational complications (p < 0.05): 94% of women with C. glabrata and 71.4% of women with C. krusei had gestational complications compared to 28.4 and 29.7% of women who did not have C. glabrata and C. krusei respectively (p-value ≤0.001). No significant associations were observed between the isolated Candida species and induced labor or recurrent UTI (Table 3).
Results of the three multiple regression models with neonatal outcomes (weight, height and Apgar score) as dependent variables are displayed in Table 4. Results revealed significant positive association between delivery time and neonatal height and significant negative association between C-section and height. Height increased 0.41 cm with 1 week increase in delivery time (p = 0.001) and decreased 0.46 cm with C-section (p = 0.002). Height also decreased with the presence of all identified Candida species. This reduction was statistically significant in both C. krusei or C. glabrata infections (Beta = − 0.46, p = 0.05 for C. albicans and Beta = − 0.77; p = 0.006). The other covariates did not yield significant associations with height. Neonatal weight had significant positive association with delivery time and significant negative association with C-section. There was 0.32 g increase in weight with an additional delivery week (p = 0.01) and 0.34 g decrease in weight with C-section (p = 0.02). Although weight decreased with the presence of Candida species (C. albicans: Beta = 0.16, C. krusei or C. glabrata: Beta = 0.43), this reduction was not statistically significant. The other covariates did not yield significant associations with weight. Apgar score did not show significant correlation with the presence of any Candida species or with any of the other independent variables.
The prevalence of Candida species causing vaginitis is pregnant women vary from one population to another. In our study, 39% of participating women were infected by Candida species. NCAC were more frequently isolated (58%) than C. albicans (42%). NCAC were also shown to increase in non vaginal clinical samples isolated from Lebanon; that was observed in a previous retrospective study published where the authors have shown that among all Candida strains isolated, C. albicans rates had decreased from 86% in 2005 to around 60% in 2014. However, the NCAC rates increased from 14% in 2005 to around 40% in 2014, comprising mainly of C. tropicalis, C. glabrata, and C. parapsilosis . Recent emergence of NCAC, such as C. glabrata and C. krusei has been seen in the post FCZ era and in settings with azole selection pressure . Worldwide, there is a variation in the distribution of Candida spp. identified from vaginal swabs and depends largely on the location as well as the population studied (Fig. 1).
Treatment of vaginal candidiasis is successfully achieved by use of azoles . NCAC related disease is less likely to respond to azole therapy, alternative treatment with AMB suppositories with or without topical azole is recommended. In the current study, isolates showed high susceptibility to AMB (97.5%) and this observation has been corroborated by studies done in various other countries including Lebanon [30, 33,34,35]. Resistance rates of C. albicans to VCZ, FCZ, and ICZ and in this study were 2.5, 10, and 12.5%, respectively, which are in contrast to earlier data from Lebanon reporting 3 to 6%, 0 to 6, and 38% resistance, respectively . However, despite high susceptibility rates against FCZ and VCZ in the previous study, their MIC90 showed an elevated trend over 10 year of study period . The increase in azole resistance in our study can be attributed to the frequent empiric prescription of FCZ for sporadic VVC, which may result in FCZ-resistant C. albicans causing recurrent VVC infection to emerge . Identification of the most common molecular mechanism of resistance among our clinical isolates would help in understanding if there is any spread of resistance gene between C. albicans and NCAC. Since through vertical or horizontal transmission, 5–30% of all colonized preterm neonates may develop invasive Candida infection [13,14,15], prophylaxis with antifungal agents in this group of patients has proven effective in preventing such an infection. However, an increase in MIC against antifungal agents may have major consequences resulting in poor outcomes and higher mortality rate among neonates with invasive candida infection.
Although treatment of asymptomatic pregnant women with Candida colonization in the genital tract is not yet recommended, some countries such as Germany have started to implement the process of screening and treatment of women found to be colonized vaginally by Candida spp. or those who present with VVC in the third trimester . In Lebanon, unlike group B streptococcus (GBS), routine screening for the presence of Candida spp. in pregnant women in the third week of gestation is not considered as part of a routine surveillance by the obstetricians. Since invasive candidiasis in neonates is becoming a serious and common cause of late onset sepsis, with mortality rates reaching as high as 25–35% , screening simultaneously for both GBS and Candida spp. in pregnant women would reduce the rate of sepsis, meningitis, oral thrush and diaper dermatitis in newborns with these organisms acquired during vaginal delivery.
It is reported that vaginitis in pregnancy is related to adverse perinatal outcome . In the current study, we aimed to find correlation between the presence of candidiasis and pregnancy outcome. Our results showed that height decreased with the presence of Candida species. This reduction was statistically significant in the presence of C. krusei or C. glabrata. However no effect was observed on the weight of the baby. This finding was consistent with a study done previously in Iran where they found no association between vaginal Candida colonization and low birth weight . The current study has also shown that Candida species cause gestational complications which is also in agreement with a previous study done in China .
Among the different studied variables which may be affected by Candida, such as gestational complications, gestational diabetes, vaginal discharge, induced labor and recurrent UTI, the present study confirmed that the presence of C. albicans was significantly associated with women with gestational diabetes and both C. krusei and C. glabrata on women with gestational complications. Future case control studies should be performed to compare the clinical outcome of pregnant women infected with any microorganism versus non infected women.
The study has potential limitations which include the lack of screening of other pathogens in pregnant women; these may have an impact on pregnancy outcomes. Case control studies should be also implemented to determine if exposure to Candida species has an association with pregnancy outcomes.
In conclusion, increasing rates of NCAC strains among pregnant women in Lebanon should be looked at as both novel and alarming. Extensive surveillance studies should be done on all clinical specimens yielding significant growth of Candida spp. and the effect of resistance pattern on ICI. As a consequence of selective pressure, emergence of drug resistance is inevitable. Therefore future studies should focus on the emergence of drug-resistant Candida strains and their frequencies. The susceptibility pattern of C. albicans to antifungal agents varies with region and would require constant monitoring of any unusual increase in resistance.
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Standardized beta coefficients
Clinical and Laboratory Standards Institute
Group B streptococcus
Non-C. albicans Candida
Neonatal intensive care unit
Coefficient of determination
Sabouraud dextrose agar
Urinary tract infections
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This study was approved by the institutional review board of Beirut Arab University under IRB# 0041-S-P-0336. A written informed consent was obtained from all eligible women before entering the study.
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Ghaddar, N., Anastasiadis, E., Halimeh, R. et al. Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon. BMC Infect Dis 20, 32 (2020). https://0-doi-org.brum.beds.ac.uk/10.1186/s12879-019-4736-2
- Candida albicans
- Pregnant women
- Antifungal susceptibility