Table 2 Sequences of the primers and probes used for the RAA assay
From: Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection
Primer/probe | Sequence (5′-3′) | Genomic position | Product size (bp) |
|---|---|---|---|
F-primer | CTTTAACAATAACCGCTGGTTTGAATATGTA | 182,540–182,570 | 164 bp |
R-primer | CTACTAAGTTCAGGTTGCTTTCAAGTTCAT | 182,703–182,674 | 164 bp |
Probe | TTGCTGGCGCTAAGTTCGTTGGTAGGGAAC [FAM-dT] C [THF] T [BHQ-dT] TTAGCGGGTACCATTA [3′-block] | 182,584–182,634 | 164 bp |