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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Diagnosis of severe scrub typhus infection by next-generation sequencing:a case report

Fig. 2

Confirmation of O. Tsutsugamushi specific amplification from plasma by next-generation sequencing. a shows the reads mapped to O. Tsutsugamushi derived from NGS data. A total of 317 reads mapped to O. Tsutsugamushi in the reference database which contains about 8000 pathogen genomes, and got a total coverage of 0.99% respectively. b shows the confirmation of O. Tsutsugamushi specific amplification by PCR. The primer was 5′-AACTGATTTTATTCAACTAATGCTGCT-3′ and 5′-TATGCCTGAGTAAGATACRTGAATRGAATT-3′. The 118 bp PCR products was detected in case sample. Lane 1: sample case, Lane 2: negative control, Lane 3: The DNA ladder was DL2000 from TAKARA. c shows the the distribution of bacterial sequences (N = 518 reads) identified in the patient’s plasma included Orientia tsutsugamushi(N = 317;61.20%), propionibacterium, staphylococcus, acinetobacter, sphingomonas, pseudomonas

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