Skip to main content

Table 1 Oligonucleotide primer sequences used for Nested PCR in this research

From: Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65

Name

Sequences(5′-3′)

Description

OUT-F

TCTGGAATGGCTGAAGAAGACG

Forward primer for the actin gene of T. vaginalis in the first stage

OUT-R

CAGGGTACATCGTATTGGTC

Reverse primer for the actin gene of T. vaginalis in the first stage

IN-F

CAGACACTCGTTATCG

Forward primer for the actin gene of T. vaginalis in the second stage

IN-R

CGGTGAACGATGGATG

Reverse primer for the actin gene of T. vaginalis in the second stage

AP65-FIP

GCCGACATAGAAGGATGGGA

CGCCCACTCAACCCAAAGGC

Forward inner primer for the AP65 gene of T. vaginalis in LAMP assay

AP65-BIP

CCTCTACTCCTCTGGCCGTACAA

ACTGTGTGGGAAACACCAT

Backward inner primer for the AP65 gene of T. vaginalis in LAMP assay

AP65-F3

CAACAGAGCACCCAGTTCTT

Forward outer primer for the AP65 gene of T. vaginalis in LAMP assay

AP65-B3

TGTGGAAGGGAGTAGCCTT

Backward outer primer for the AP65 gene of T. vaginalis in LAMP assay