Table 1 Primary polyclonal antibodies, immunogens used for the production of antibodies, target antigens, the name used in the study, and dilutions used for immunohistochemistry
Antibody | Immunogen | Target antigens | Study name | Dilution |
|---|---|---|---|---|
Anti-MPT64 | Antigen extracted and purified from the 5 weeks old culture filtrate of M. tuberculosis with minimal lysis. The term MPT was introduced by Nagai et al. for the designation of proteins purified from M.tuberculosis. The number is based on their relative mobility in 7.7% polyacrylamide gel electrophoresis gels at a running pH of 9.5 [29]. | MPT64 (Rv1980c). Secreted antigen specific for M.tuberculosis complex organisms. Absent from most of atypical mycobacteria. | secreted antigen | 1:50 |
Anti-Antigen 60 | Cell wall antigens of M.tuberculosis. The number is based on their relative mobility as mentioned above [29] | Major M.tuberculosis cell wall antigens. | Cell wall antigens | 1:100 |
Anti- Lipoarabinomannan (LAM) | LAM | LAM, the primary component of M.tuberculosis cell wall. | LAM | 1:100 |
Anti-Bacille Calmette- Gue’rin (BCG) | Bacterial sonicates of BCG containing both secreted and cell wall antigens. | Most of the secreted and cell wall antigens of the M. tuberculosis based on the similarity between M.tuberculosis and BCG. | Heterogeneous antigens | 1:2000 |