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Table 1 Conditions and primers used for PCR, amplified product base pair size, and references used for PCR in this study

From: First identification of Anaplasma phagocytophilum in both a biting tick Ixodes nipponensis and a patient in Korea: a case report

PCR assay

Name of primer seq (5′-3′)

PCR conditions

Product Size (bp)

Reference

Denaturation

Annealing

Extension

Cycles

(°C/sec)

(°C/sec)

(°C/sec)

 

16 s rRNA PCR for tick identification

16S - 1-F (CTGCTCAATGAATATTTAAATTGC)

95/45

55/60

72/90

40

450

(https://shire.science.uq.edu.au/parasites/arachnids/ticks/ticks-identification.php)

16S-1-R (CGGTCTAAACTCAGATCATGTAGG)

    

ankA N-PCR for Anaplasma phagocytophilum

ANK-F1 (GAAGAAATTACAACTCCTGAAG)

95/30

53/30

72/60

35

705

[7]

ANK-R1 (CAGCCAGATGCAGTAACGTG)

    

ANK-F2 (TTGACCGCTGAAGCACTAAC)

95/30

52/30

72/60

5

664

ANK-R2 (ACCATTTGCTTCTTGAGGAG)

95/30

54/30

72/60

25

groEL N-PCR for Anaplasma phagocytophilum

HS1 TGGGCTGGTA(A/C)TGAAAT

94/60

48/120

70/90

3

1300

[8]

HS6 CCICCIGGIACIA(C/T)ACCTTC

88/60

52/120

70/90

37

HS43 AT(A/T)GC(A/T)AA(G/A)GAAGCATAGTC

94/60

48/120

70/90

3

528

HS45 ACTTCACG(C/T)(C/T)TCATAGAC

88/60

55/120

70/90

37

ompA N-PCR for Rickettsia spp.

R190.70F (ATGGCGAATATTTCTCCAAAAA)

94/30

50/30

72/60

40

634

[9]

RR190.701R (GTTCCGTTAATGGCAGCATCT)

    

R190.70F (ATGGCGAATATTTCTCCAAAAA)

94/30

51/30

72/30

5

535

RR190.602R (AGTGCAGCATTCGCTCCCCCT)

94/30

55/30

72/30

30

56 kDa N-PCR for Orientia tsutsugamushi

56BO-144F (YGYAGAATCTRCTCGCTTGG)

94/60

60/60

72/60

35

1250

In this study

56BO-1395R (agctaMccctRcaccaaBac)

    

56BO-406F (CCWCCTCARCCTACTAtrTGC)

94/30

61/30

72/45

30

680

56BO-1088R (gcWgctgctRctgcttcttg)

    

pyrG N-PCR for Borrelia spp.

pyrG-1F (ATTGCAAGTTCTGAGAATA)

94/20

45/30

72/30

30

801

[10]

pyrg-1R (CAAACATTACGAGCAAATTC)

    

pyrG-2F (GATATGGAAAATATTTTATTTATTG)

95/30

45/30

72/30

5

707

pyrg-2R (AAACCAAGACAAATTCCAAG)

95/30

47/30

72/30

5

 

95/30

49/30

72/30

25

RT-PCR for SFTSV

MF3 (GATGAGATGGTCCATGCTGATTCT)

95/20

58/40

72/30

35

560

[11]

MR2 (CTCATGGGGTGGAATGTCCTCAC)