PCR assay | Name of primer seq (5′-3′) | PCR conditions | Product Size (bp) | Reference | |||
---|---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | Cycles | ||||
(°C/sec) | (°C/sec) | (°C/sec) | |||||
16 s rRNA PCR for tick identification | 16S - 1-F (CTGCTCAATGAATATTTAAATTGC) | 95/45 | 55/60 | 72/90 | 40 | 450 | (https://shire.science.uq.edu.au/parasites/arachnids/ticks/ticks-identification.php) |
16S-1-R (CGGTCTAAACTCAGATCATGTAGG) | |||||||
ankA N-PCR for Anaplasma phagocytophilum | ANK-F1 (GAAGAAATTACAACTCCTGAAG) | 95/30 | 53/30 | 72/60 | 35 | 705 | [7] |
ANK-R1 (CAGCCAGATGCAGTAACGTG) | |||||||
ANK-F2 (TTGACCGCTGAAGCACTAAC) | 95/30 | 52/30 | 72/60 | 5 | 664 | ||
ANK-R2 (ACCATTTGCTTCTTGAGGAG) | 95/30 | 54/30 | 72/60 | 25 | |||
groEL N-PCR for Anaplasma phagocytophilum | HS1 TGGGCTGGTA(A/C)TGAAAT | 94/60 | 48/120 | 70/90 | 3 | 1300 | [8] |
HS6 CCICCIGGIACIA(C/T)ACCTTC | 88/60 | 52/120 | 70/90 | 37 | |||
HS43 AT(A/T)GC(A/T)AA(G/A)GAAGCATAGTC | 94/60 | 48/120 | 70/90 | 3 | 528 | ||
HS45 ACTTCACG(C/T)(C/T)TCATAGAC | 88/60 | 55/120 | 70/90 | 37 | |||
ompA N-PCR for Rickettsia spp. | R190.70F (ATGGCGAATATTTCTCCAAAAA) | 94/30 | 50/30 | 72/60 | 40 | 634 | [9] |
RR190.701R (GTTCCGTTAATGGCAGCATCT) | |||||||
R190.70F (ATGGCGAATATTTCTCCAAAAA) | 94/30 | 51/30 | 72/30 | 5 | 535 | ||
RR190.602R (AGTGCAGCATTCGCTCCCCCT) | 94/30 | 55/30 | 72/30 | 30 | |||
56 kDa N-PCR for Orientia tsutsugamushi | 56BO-144F (YGYAGAATCTRCTCGCTTGG) | 94/60 | 60/60 | 72/60 | 35 | 1250 | In this study |
56BO-1395R (agctaMccctRcaccaaBac) | |||||||
56BO-406F (CCWCCTCARCCTACTAtrTGC) | 94/30 | 61/30 | 72/45 | 30 | 680 | ||
56BO-1088R (gcWgctgctRctgcttcttg) | |||||||
pyrG N-PCR for Borrelia spp. | pyrG-1F (ATTGCAAGTTCTGAGAATA) | 94/20 | 45/30 | 72/30 | 30 | 801 | [10] |
pyrg-1R (CAAACATTACGAGCAAATTC) | |||||||
pyrG-2F (GATATGGAAAATATTTTATTTATTG) | 95/30 | 45/30 | 72/30 | 5 | 707 | ||
pyrg-2R (AAACCAAGACAAATTCCAAG) | 95/30 | 47/30 | 72/30 | 5 | |||
95/30 | 49/30 | 72/30 | 25 | ||||
RT-PCR for SFTSV | MF3 (GATGAGATGGTCCATGCTGATTCT) | 95/20 | 58/40 | 72/30 | 35 | 560 | [11] |
MR2 (CTCATGGGGTGGAATGTCCTCAC) |