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Table 2 Characteristics of pncA gene mutations in MDR-TB patient isolates

From: Value of pyrazinamide for composition of new treatment regimens for multidrug-resistant Mycobacterium tuberculosis in China

Serial numberAmino acids changesPhenotypic pyrazinamide susceptibility testing results
susceptibleresistanttotal
1A(−11)G033
2Met1Thr224
3Leu4Trp011
4Ile5Serb011
5Val9Alab011
6Gln10Pro044
7Gln10stop101
8Asp12Ala011
9Asp12Asn213
10Asp12Glu011
11His137Argb112
12Cys14Glya101
13Cys14Tyrb011
14Gly17Asp011
15Gly17Serb011
16Leu27Pro022
17Tyr34Ser011
18Val44Gly011
19Thr160Pro011
20Thr47Alaa112
21Lys48Arg101
22Lys48Glu033
23Pro54Leu022
24His57Glnb022
25Pro62Leu011
26Pro62Pro(synonymous)a101
27Asp63Tyr/Thr142Lysb011
28Ser164Prob011
29Ser65Ser(synonymous)a101
30Trp68Gly011
31Pro69Leu101
32His71Pro011
33Thr76Pro033
34Leu85Pro022
35Phe94Ser022
36Lys96Arg011
37Lys96Metb011
38Lys96Thr011
39Gly97Ser011
40Ser66Leu101
41Ala102Pro011
42Tyr103Cys202
43Tyr103Ser101
44Leu116Arg011
45Trp119Argb011
46Gln122stopb011
47Val125Gly011
48Val130Alaa101
49Val130Met101
50Val139Gly011
51Gly132Asp033
52Ile133Thr011
53Asp136Asn011
54Asp136Gly011
55Cys138Trpb022
56Val139Leu022
57Arg140Glyb011
58Gln141Pro022
59Thr100Prob022
60Thr142Ala022
61Ala146Thrb022
62Asn149Val011
63Arg154Trpb011
64Val155Met101
65Val157Glyb011
66Thr142Met011
67Ala161Glyb011
68Gly162Val011
69Ala171Vala202
70Leu172Prob011
71Glu173Glu(synonymous)a101
72Glu174Glyb011
73Met175Ile022
74Met175Val022
75Leu182Serb011
76Frameshiftb11112
Wild type16935204
Total192132324
  1. Note: a This synonymous single-nucleotide polymorphism (sSNP), or nonsynonymous single-nucleotide polymorphism (nSNP) not relating to pyrazinamide resistance (18) was excluded for calculating the concordance between genotypic mutations and phenotypic susceptible results
  2. b These mutations and all frameshift changes (insertions or deletions) relating to pyrazinamide resistance were first found in this study