Skip to main content

Table 2 Characteristics of pncA gene mutations in MDR-TB patient isolates

From: Value of pyrazinamide for composition of new treatment regimens for multidrug-resistant Mycobacterium tuberculosis in China

Serial number

Amino acids changes

Phenotypic pyrazinamide susceptibility testing results

susceptible

resistant

total

1

A(−11)G

0

3

3

2

Met1Thr

2

2

4

3

Leu4Trp

0

1

1

4

Ile5Serb

0

1

1

5

Val9Alab

0

1

1

6

Gln10Pro

0

4

4

7

Gln10stop

1

0

1

8

Asp12Ala

0

1

1

9

Asp12Asn

2

1

3

10

Asp12Glu

0

1

1

11

His137Argb

1

1

2

12

Cys14Glya

1

0

1

13

Cys14Tyrb

0

1

1

14

Gly17Asp

0

1

1

15

Gly17Serb

0

1

1

16

Leu27Pro

0

2

2

17

Tyr34Ser

0

1

1

18

Val44Gly

0

1

1

19

Thr160Pro

0

1

1

20

Thr47Alaa

1

1

2

21

Lys48Arg

1

0

1

22

Lys48Glu

0

3

3

23

Pro54Leu

0

2

2

24

His57Glnb

0

2

2

25

Pro62Leu

0

1

1

26

Pro62Pro(synonymous)a

1

0

1

27

Asp63Tyr/Thr142Lysb

0

1

1

28

Ser164Prob

0

1

1

29

Ser65Ser(synonymous)a

1

0

1

30

Trp68Gly

0

1

1

31

Pro69Leu

1

0

1

32

His71Pro

0

1

1

33

Thr76Pro

0

3

3

34

Leu85Pro

0

2

2

35

Phe94Ser

0

2

2

36

Lys96Arg

0

1

1

37

Lys96Metb

0

1

1

38

Lys96Thr

0

1

1

39

Gly97Ser

0

1

1

40

Ser66Leu

1

0

1

41

Ala102Pro

0

1

1

42

Tyr103Cys

2

0

2

43

Tyr103Ser

1

0

1

44

Leu116Arg

0

1

1

45

Trp119Argb

0

1

1

46

Gln122stopb

0

1

1

47

Val125Gly

0

1

1

48

Val130Alaa

1

0

1

49

Val130Met

1

0

1

50

Val139Gly

0

1

1

51

Gly132Asp

0

3

3

52

Ile133Thr

0

1

1

53

Asp136Asn

0

1

1

54

Asp136Gly

0

1

1

55

Cys138Trpb

0

2

2

56

Val139Leu

0

2

2

57

Arg140Glyb

0

1

1

58

Gln141Pro

0

2

2

59

Thr100Prob

0

2

2

60

Thr142Ala

0

2

2

61

Ala146Thrb

0

2

2

62

Asn149Val

0

1

1

63

Arg154Trpb

0

1

1

64

Val155Met

1

0

1

65

Val157Glyb

0

1

1

66

Thr142Met

0

1

1

67

Ala161Glyb

0

1

1

68

Gly162Val

0

1

1

69

Ala171Vala

2

0

2

70

Leu172Prob

0

1

1

71

Glu173Glu(synonymous)a

1

0

1

72

Glu174Glyb

0

1

1

73

Met175Ile

0

2

2

74

Met175Val

0

2

2

75

Leu182Serb

0

1

1

76

Frameshiftb

1

11

12

–

Wild type

169

35

204

–

Total

192

132

324

  1. Note: a This synonymous single-nucleotide polymorphism (sSNP), or nonsynonymous single-nucleotide polymorphism (nSNP) not relating to pyrazinamide resistance (18) was excluded for calculating the concordance between genotypic mutations and phenotypic susceptible results
  2. b These mutations and all frameshift changes (insertions or deletions) relating to pyrazinamide resistance were first found in this study