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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: The S68G polymorphism is a compensatory mutation associated with the drug resistance mutation K65R in CRF01_AE strains

Fig. 3

Growth competition assay for the K65R and K65R/S68G infectious clones. The infectious clones of K65R and K65R/S68G obtained from patients were co-cultured for 13 days in PBMCs with the following input ratios for K65R vs. K65R/S68G: 4:6 (a), 1:1 (b), and 9:1 (c). Viral RNA from culture supernatants was used to amplify target regions of pol genes, and these regions were subsequently sequenced. ChromatQuan (http://indra.mullins.microbiol.washington.edu/cgi-bin/chromatquant.cgi) was used to calculate the viral percentages at each time point. The average of three independent experiments is shown, with error bars representing the SD

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BMC Infectious Diseases

ISSN: 1471-2334

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