Skip to main content

Table 3 Test accuracy for various routine diagnostic tests, Xpert Ultra and the MPT64 test using a composite reference standard

From: Diagnosis of extrapulmonary tuberculosis using the MPT64 antigen detection test in a high-income low tuberculosis prevalence setting

 

Test performed on number of samples

Sensitivity %

(95% CI)

Specificity %

(95% CI)

Overall accuracy

%

All samples (n = 274)

 Microscopy

166

22 (9–42)

95 (90–98)

83

 Culture

252

62 (41–80)

100 (98–100)

96

 PCR (1-step PCR and n-PCR pooled)

87

44 (26–65)

100 (94–100)

83

 Xpert Ultra

48

57 (18–90)

98 (87–100)

92

 MPT64 test

274

32 (17–51)

84 (79–88)

78

Lymph node biopsies (n = 24)

 Microscopy

19

17 (0–64)

92 (64–100)

68

 Culture

20

33 (4–78)

100 (77–100)

80

 PCR (1-step PCR and n-PCR pooled)

15

29 (4–71)

100 (63–100)

67

 Xpert Ultra

2

N/A

100 (16–100)

 MPT64 test

24

43 (10–82)

100 (80–100)

83

All biopsies (n = 88)

 Microscopy

63

20 (4–48)

94 (83–99)

76

 Culture

68

50 (23–77)

100 (93–100)

90

 PCR (1-step PCR and n-PCR pooled)

57

37 (16–62)

100 (91–100)

79

 Xpert Ultra

10

N/A

90 (56–100)

 MPT64 test

88

37 (16–62)

99 (92–100)

85

Lymph node aspirates (n = 11)

 Microscopy

9

43 (10–82)

50 (1–99)

44

 Culture

9

71 (29–96)

100 (16–100)

78

 PCR (1-step PCR and n-PCR pooled)

9

67 (22–96)

100 (29–100)

78

 Xpert Ultra

5

75 (19–99)

100 (3–100)

80

 MPT64 test

11

29 (4–71)

50 (7–93)

36

 Pus samples (n = 15)

 Microscopy

15

0 (0–84)

77 (46–95)

67

 Culture

15

50 (1–99)

100 (75–100)

93

 PCR (1-step PCR and n-PCR pooled)

6

100 (3–100)

100 (48–100)

100

 Xpert Ultra

10

50 (1–99)

100 (63–100)

90

 MPT64 test

15

50 (1–99)

69 (39–91)

67

Fluid samples (n = 160)

 Microscopy

79

0 (0–71)

100 (95–100)

96

 Culture

160

100 (29–100)

100 (98–100)

100

 PCR (1-step PCR and n-PCR pooled)

15

0 (0–98)

100 (77–100)

93

 Xpert Ultra

23

0 (0–98)

100 (85–100)

96

 MPT64 test

160

0 (0–71)

80 (72–86)

78

  1. Abbreviations: PCR, polymerase chain reaction; CI, confidence interval