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Table 1 Primers used in the RT-PCR assay for amplification of the E protein gene of DENV

From: Revisiting the dengue epidemic of 2011 in Paraguay: molecular epidemiology of dengue virus in the Asuncion metropolitan area

Virus

Primers

Sequence (5ʹ- 3ʹ)

Position

GenBank Nº

References

DENV-1

d1s3

AAACGTTCCGTSGCACTGGC*

704–709

MT447147

[22]

 

d1a17

CCAATGGCYGCTGAYAGTCT*

2540–2559

  

DENV-2

ES(D2)B

GGCATACACCATAGGAACGAC

858–878

MN018365

Designed in this study

 

EC(D2)B

AGGGGATTCTGGTTGGAACTT

2518–2538

  

DENV-3

ES(D3)

GCCCTATTTCTTGCCCATTACA

845–866

MH823209

[13]

 

EC(D3)

CCGCACACTCCATTCTCCCAA

2560–2580

  
  1. *S = G + C; Y = C + T