Table 4 Amplification results for each gene in specimens from confirmed cases and screening cases
From: Clinical and epidemiologic characteristics of inconclusive results in SARS-CoV-2 RT-PCR assays
| Â | Specimens from confirmed cases | Specimens from screening cases | ||
|---|---|---|---|---|
Detection rate (%) | Cycle threshold (SD) | Detection rate (%) | Cycle threshold (SD) | |
E gene | ||||
 Allplex (initial) | 5/125 (4.0%) | 36.0 (2.1) | 1/40 (2.5%) | 35.7 ( −) |
 Allplex (re-test) | 19/124 (15.3%) | 34.6 (1.5) | 0/36 (0%) | N/A |
 Real-Q | 60/124 (48.4%) | 36.4 (1.1) | 0/36 (0%) | N/A |
RdRP gene | ||||
 Allplex (initial) | 45/125 (36.0%) | 35.8 (1.7) | 7/40 (17.5%) | 38.0 (1.6) |
 Allplex (re-test) | 52/124 (41.9%) | 35.6 (1.6) | 4/36 (11.1%) | 36.6 (2.4) |
 Real-Q | 54/124 (43.5%) | 36.2 (1.3) | 3/36 (8.3%) | 32.0 (4.8) |
 BioCore | 55/124 (44.4%) | 37.1 (1.7) | 2/36 (5.6%) | 38.5 (0.8) |
N gene | ||||
 Allplex (initial) | 123/125 (98.4%) | 37.3 (1.6) | 37/40 (92.5%) | 38.4 (1.2) |
 Allplex (re-test) | 92/124 (74.2%) | 37.1 (1.6) | 15/36 (41.7%) | 37.5 (1.3) |
 BioCore | 11/124 (8.9%) | 38.3 (0.7) | 0/36 (0%) | N/A |
 DiaPlexQ | 85/124 (68.5%) | 37.3 (1.3) | 0/36 (0%) | N/A |
ORF1a | ||||
 DiaPlexQ | 81/124 (65.3%) | 37.3 (1.2) | 0/36 (0%) | N/A |