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Table 2 Comparison of Real-time PCR and Nested PCR methods in diagnosing of toxoplasmosis in retinochoroiditis patients

From: Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients

Genes MAG-1 SAG-4 B1
No Real-time PCR PCR Real-time PCR PCR Real-time PCR PCR
Samples P S B P S B P S B P S B P S B P S B
1  +   + 
2  + 
3  + 
4    + 
5  +   + 
6  +  * +   +   +   +   +   +   + 
7  +   +   + 
8  +   + 
9  +   + 
10  +   +   +   +   +   +   +   + 
Total 3 0 2 3 0 0 5 0 2 3 0 0 9 0 2 1 0 0
  1. P = peripheral blood mononuclear cells (PBMCs), S = Serum, B = Blood, PCR: Polymerase Chain Reaction
  2. *In blood samples that nested PCR were negative real-time were positive