From: HIV-1 Tat amino acid residues that influence Tat-TAR binding affinity: a scoping review
Tat amino acid position | Mutation | Binding affinity | Percentage increase/decrease in Tat-TAR binding affinity | Technique | References |
---|---|---|---|---|---|
5 | R5K | 6.7 × 10−7 M | 70% decrease | Surface plasmon resonance (SPR) | [86] |
49 | K49A | 4 × 10−6 M | 1900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] |
50 | K50A | 2 × 10–5 M | 9900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] |
51 | K51A | 4 × 10−6 M | 1900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] |
52 | R52A | 10 × 10−9 M | 40% decrease | Electrophoretic mobility shift assay (EMSA) | [69] |
53 | R53A | 12 X 10−9 M | 50% decrease | EMSA | [69] |
K53A | 4 × 10−6 M | 1900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] | |
54 | Q54A | 5 X 10−9 M | 20% increase | EMSA | [69] |
8 × 10−8 M | 12.5% decrease | Fluorescence resonance energy transfer (FRET), MALDI-TOFMS, Fluorescence binding assay | [82] | ||
K54A | 2 × 10–5 | 1900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] | |
55 | R55A | 12 × 10−9 M | 50% decrease | EMSA | [69] |
56 | R56A | 12 × 10−9 M | 50% decrease | EMSA | [69] |
K56A | 4 × 10−6 M | 1900% decrease | Absorption spectroscopy, Gel shift assays, CD Spectroscopy | [71] |