Gene | Primer and probe sequence (5′to3′) | Product length(bp) | GenBank |
---|---|---|---|
EV71 | (R) CCCGCTCTGCTGAAGAAACT | 89 | AF302996 |
 | (F)AGTGATGAGAGTATGATTGAGACACG |  |  |
 | (P) TCGCACAGCACAGCTGAGACCACTC |  |  |
β-actin | (R) CAAGTACTCCGTGTGGATCG | 90 | NM_001101.3 |
 | (F) GGATGCAGAAGGAGATCACTG |  |  |
 | (P) CCCTGGCACCCAGCACAATGA |  |  |
RIG-1 | (R) CCTCTGCACTGTTGCTCAGGAC | 192 | NM_004585.3 |
 | (F) CTCTTGGCTTCGAGATGGCTTC |  |  |
MDA5 | (R) ATTGGTGACGAGACCATAACGGATA | 196 | NM_022168.2 |
 | (F) AGGAGTCAAAGCCCACCATCTG |  |  |
TLR2 | (R) CACAAAGTATGTGGCATTGTCCAG | 158 | NM_003264.3 |
 | (F) GTGTTGCAAGCAGGATCCAAAG |  |  |
TLR3 | (R) AGTGCCGTCTATTTGCCACACA | 181 | NM_003265.2 |
 | (F) AACAGTGCACTTGGTGGTGGAG |  |  |
TLR4 | (R) ATGCGGACACACACACTTTCAAATA | 143 | NM_138554.3 |
 | (F) TTGAGCAGGTCTAGGGTGATTGAAC |  |  |
TLR6 | (R) AGCCTTCAGCTTGTGGTACTTGTTG | 138 | NM_006068.2 |
 | (F) CAGAGTGAGTGGTGCCATTACGA |  |  |
TLR7 | (R) TCTTCAACCAGACCTCTACATTCCA | 172 | NM_016562.3 |
 | (F) GGAACATCCAGAGTGACATCACAG |  |  |
TLR8 | (R) GATTGCTGCACTCTGCAATAACTGA | 196 | NM_138636.3 |
 | (F) GCTGCTGCAAGTTACGGAATGA |  |  |
TLR9 | (R) CAGGGCCTTCAGCTGGTTTC | 97 | NM_017442.2 |
 | (F) CCGTGACAATTACCTGGCCTTC |  |  |
TLR10 | (R) TGTGTTGCAAGATAATTCGTGGAGA | 103 | NM_001017388.1 |
 | (F) CATGATGGTTGGATGGTCAGATTC |  |  |
β-actin | (R) CTAAGTCATAGTCCGCCTAGAAGCA | 186 | NM_001101.3 |
 | (F) TGGCACCCAGCACAATGAA |  |  |