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Figure 1 | BMC Infectious Diseases

Figure 1

From: Development of real-time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV-8 lytic and latent genes

Figure 1

NASBA amplification reaction NASBA amplification reaction with the P1 (anti-sense) – P2 (sense) oligonucleotide primer set. The overhang on the P1 encodes the promoter sequence for the T7 RNA polymerase. A molecular beacon with a fluorophore and a quencer with the NASBA amplification reaction generates a real-time detection system.

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BMC Infectious Diseases

ISSN: 1471-2334

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