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Table 2 Sequences of the primers and beacons for each of the assays

From: Development of real-time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV-8 lytic and latent genes

 

Primer P1 Anti-sense primer

Primer P2 Sense primer

Beacon Stem-loop in lowercase italics

ORF73

AG AGA CAA TAC ACA TAT ACA CAA TAA G

GAA AGG ATG GAA GAC GAG ATC CA

gca cgc AGG AGT AAA GGC AGG CCC CGT GTC gc g tgc

vGCR

AA CGA GGT TAC TGC CAG ACC CAC GT

CAG GCG GAA GGT AAG GGG GGT GA

gca cgc TGA TTG TTG CTG TGG TGC TGC T gcg tcg

vBcl-2

AA GCG AAA CCA CTG GGG TCC GAT TG

GTG AGA TTT CAC AGC ACC ACC GGT A

gca cgc TGA CCT TTG GCA GTT TTG TGG CC gcg tgc

vIL-6

AG AAC ATA AAA CGA AGC AAA GTG TCT CA

GGA AAA TCA GTG ATA AAC GTG GA

gca cgc GGA AGA GCT CAA TAA GCT GAC TA gcg tgc

U1A

AG AGG CCC GGC ATG TGG TGC ATA A

CAG TAT GCC AAG ACC GAC TCA GA

cgt acg AGA AGA GGA AGC CCA AGA GCC A cgt acg

  1. The 3' anti-sense primer is elongated with T7-promotor recognition sequence: AAT TCT AAT ACG ACT CAC TAT AGG G