Table 1 Cycling protocol for HBD-1, HBD-2 and GAPDH RT-PCR
From: Expression of human beta-defensins 1 and 2 in kidneys with chronic bacterial infection
Step | Denaturation time/temperature | Annealing time/temperature | Extension time/temperature | Number of cycles |
|---|---|---|---|---|
HBD-1 | ||||
1 | 1 5 min/95°C | 30 sec/55°C | 30 sec/72°C | 1 |
2 | 30 sec/95°C | 30 sec/55°C | 30 sec/72°C | 28 |
3 | 30 sec/95°C | 30 sec 55°C | 10 min/72°C | 1 |
HBD-2 | ||||
1 | 1 5 min/95°C | 30 sec/60°C | 30 sec/72°C | 1 |
2 | 30 sec/95°C | 30 sec/60°C | 30 sec/72°C | 28 |
3 | 30 sec/95°C | 30 sec/60°C | 10 min/72°C | 1 |
2 GAPDH | ||||
1 | 5 min/95°C | 30 sec/55°C or 60°C | 30 sec/72°C | 1 |
2 | 30 sec/95°C | 30 sec/55°C or 60°C | 30 sec/72°C | 28 |
3 | 30 sec/95°C | 30 sec/55°C or 60°C | 10 min/72°C | 1 |