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Table 2 Studies without controls of Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells

From: Association of circulating Chlamydia pneumoniaeDNA with cardiovascular disease: a systematic review

Reference Country Study group Prevalence (%) Blood sampling DNA Extraction PCRa target
Blasi, 1999 [30] Italy Aortic aneurysm 19 of 41 (46.3) 8 mL CPTc tube Phenol-chloroform Nested MOMPd
Blasi, 2000 (abstract) Italy COPDe 10 of 21 (47.6) 8 mL CPT tube Not stated Not stated
Bodetti 2000 [34] Australia Blood donors 10 of 60 (16.7) 9 mL EDTA Not stated Nested MOMP
Haranaga, 2001 [36] USA Blood donors 21 of 237 (8.9) 5 mL EDTA QIAamp DNA extraction Touchdown 16S rDNA
Iliescu, 2000 [33] Canada Dialysis 33 of 55 (60.0) 10 mL heparin QIAamp DNA extraction 53 kDa protein
Maass, 2000 [29] Germany Unstable angina, myocardial infarction 65 of 238 (27.3) 8 mL EDTAb, CD14+ cell selection QIAamp DNA extraction Nested pstI cloned fragment
Rassu, 2001 [35] Italy Blood donors 78 of 169 (46.2) 8 mL EDTA Phenol-chloroform Nested MOMP
Smieja, 2002 [32] Canada COPD 24 of 100 (24.0) 8 mL CPT tube QIAamp DNA extraction Nested MOMP
Taylor-Robinson, 2000 [31] United Kingdom Peripheral vascular disease Not stated (47.0) Not stated Not stated Not stated
  1. a PCR, polymerase chain reaction. b EDTA, ethylene diamine tetra-acetic acid c CPT, BD cell preparation tube (BD Vacutainer Systems, Franklin Lakes NJ, USA) d MOMP, major outer membrane protein e COPD, chronic obstructive pulmonary disease