Table 2 Studies without controls of Chlamydia pneumoniae DNA detection in peripheral blood mononuclear cells
From: Association of circulating Chlamydia pneumoniaeDNA with cardiovascular disease: a systematic review
Reference | Country | Study group | Prevalence (%) | Blood sampling | DNA Extraction | PCRa target |
|---|---|---|---|---|---|---|
Blasi, 1999 [30] | Italy | Aortic aneurysm | 19 of 41 (46.3) | 8 mL CPTc tube | Phenol-chloroform | Nested MOMPd |
Blasi, 2000 (abstract) | Italy | COPDe | 10 of 21 (47.6) | 8 mL CPT tube | Not stated | Not stated |
Bodetti 2000 [34] | Australia | Blood donors | 10 of 60 (16.7) | 9 mL EDTA | Not stated | Nested MOMP |
Haranaga, 2001 [36] | USA | Blood donors | 21 of 237 (8.9) | 5 mL EDTA | QIAamp DNA extraction | Touchdown 16S rDNA |
Iliescu, 2000 [33] | Canada | Dialysis | 33 of 55 (60.0) | 10 mL heparin | QIAamp DNA extraction | 53 kDa protein |
Maass, 2000 [29] | Germany | Unstable angina, myocardial infarction | 65 of 238 (27.3) | 8 mL EDTAb, CD14+ cell selection | QIAamp DNA extraction | Nested pstI cloned fragment |
Rassu, 2001 [35] | Italy | Blood donors | 78 of 169 (46.2) | 8 mL EDTA | Phenol-chloroform | Nested MOMP |
Smieja, 2002 [32] | Canada | COPD | 24 of 100 (24.0) | 8 mL CPT tube | QIAamp DNA extraction | Nested MOMP |
Taylor-Robinson, 2000 [31] | United Kingdom | Peripheral vascular disease | Not stated (47.0) | Not stated | Not stated | Not stated |