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Table 1 Summary of NoV primers used

From: Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation

Real-time PCR (polymerase gene)

Primer

Location

Oligonucleotide sequence (5' - 3')

Reference

NV p110a

4865 - 4883

ACDATYTCATCATCACCATA

18

NV p36a

4487 - 4505

ATAAAAGTTGGCATGAACA

15

NV NIb

4495 - 4515

GAATTCCATCGCCCACTGGCT

17

Nested PCR (polymerase gene)

Primer

Location

Oligonucleotide sequence (5' - 3')

Reference

Primer 32b

4226 - 4245

ATGAATATGAATGAAGATGG

23

Primer 36a

4980 - 4961

ATTGGTCCTTCTGTTTTGTC

23

Primer 35a

4890 - 4871

GTTGACACAATCTCATCATC

23

Primer 33b

4280 - 4299

TACCACTATGATGCAGATTA

23

  1. Annealing site refer to aGenBank accession no. M87661 and bGenBank no X86557